ID   CAPSP_ADE12             Reviewed;         497 AA.
AC   P36716;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   02-JUN-2021, entry version 74.
DE   RecName: Full=Penton protein {ECO:0000255|HAMAP-Rule:MF_04052};
DE            Short=CP-P {ECO:0000255|HAMAP-Rule:MF_04052};
DE   AltName: Full=Penton base protein {ECO:0000255|HAMAP-Rule:MF_04052};
DE   AltName: Full=Protein III {ECO:0000255|HAMAP-Rule:MF_04052};
GN   Name=L2 {ECO:0000255|HAMAP-Rule:MF_04052};
OS   Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Rowavirales; Adenoviridae; Mastadenovirus; Human mastadenovirus A.
OX   NCBI_TaxID=28282;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8254750; DOI=10.1128/jvi.68.1.379-389.1994;
RA   Sprengel J., Schmitz B., Heuss-Neitzel D., Zock C., Doerfler W.;
RT   "Nucleotide sequence of human adenovirus type 12 DNA: comparative
RT   functional analysis.";
RL   J. Virol. 68:379-389(1994).
CC   -!- FUNCTION: Major capsid protein that self-associates to form penton base
CC       pentamers, each in the shape of a pentagon, situated at the 12 vertices
CC       of the pseudo T=25 capsid. Involved in virus secondary attachment to
CC       host cell after initial attachment by the fiber protein. Binds host
CC       integrin heterodimer ITGAV-ITGB5 (alphaV-beta5) thereby triggering
CC       clathrin-mediated endocytosis of virions. Mediates initial virus
CC       attachment to CXADR-negative cells. Binding to integrins ITGAV-ITGB5
CC       also seems to induce macropinocytosis uptake of the virus. As the virus
CC       enters the host cell, penton proteins are shed concomitant with virion
CC       acidification in the endosome. {ECO:0000255|HAMAP-Rule:MF_04052}.
CC   -!- SUBUNIT: Interacts (via the cell attachment site RGD) with host
CC       heterodimer ITGAV-ITGB5; this interaction promotes virus
CC       internalization. Interacts with host WWP1 and WWP2. Interacts with the
CC       fiber protein (via N-terminal tail region). Interacts with the capsid
CC       vertex protein; this interaction binds the penton base to neighboring
CC       peripentonal hexons. {ECO:0000255|HAMAP-Rule:MF_04052}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04052}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04052}. Note=Located at each vertex
CC       of the virion. Present in 60 copies per virion. {ECO:0000255|HAMAP-
CC       Rule:MF_04052}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000255|HAMAP-Rule:MF_04052}.
CC   -!- DOMAIN: The cell attachment RGD motif is exposed at the virion surface
CC       and is involved in binding to the integrin heterodimer ITGAV-ITGB5.
CC       {ECO:0000255|HAMAP-Rule:MF_04052}.
CC   -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC       are produced by alternative splicing and alternative polyadenylation of
CC       the same gene giving rise to non-overlapping ORFs. A leader sequence is
CC       present in the N-terminus of all these mRNAs and is recognized by the
CC       viral shutoff protein to provide expression although conventional
CC       translation via ribosome scanning from the cap has been shut off in the
CC       host cell. {ECO:0000255|HAMAP-Rule:MF_04052}.
CC   -!- SIMILARITY: Belongs to the adenoviridae penton family.
CC       {ECO:0000255|HAMAP-Rule:MF_04052}.
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DR   EMBL; X73487; CAA51887.1; -; Genomic_DNA.
DR   PIR; S33938; S33938.
DR   RefSeq; NP_040920.1; NC_001460.1.
DR   SMR; P36716; -.
DR   GeneID; 1460868; -.
DR   KEGG; vg:1460868; -.
DR   Proteomes; UP000004993; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039623; C:T=25 icosahedral viral capsid; IEA:UniProtKB-UniRule.
DR   GO; GO:0005198; F:structural molecule activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0075512; P:clathrin-dependent endocytosis of virus by host cell; IEA:UniProtKB-KW.
DR   GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04052; ADV_CAPSP; 1.
DR   InterPro; IPR002605; Adeno_Penton_B.
DR   Pfam; PF01686; Adeno_Penton_B; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Clathrin-mediated endocytosis of virus by host;
KW   Host nucleus; Host-virus interaction; Late protein;
KW   T=25 icosahedral capsid protein; Viral attachment to host cell;
KW   Viral penetration into host cytoplasm; Virion; Virus endocytosis by host;
KW   Virus entry into host cell.
FT   CHAIN           1..497
FT                   /note="Penton protein"
FT                   /id="PRO_0000221874"
FT   MOTIF           293..295
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04052"
SQ   SEQUENCE   497 AA;  56394 MW;  0524D989F5A9ED13 CRC64;
     MRRAVELQTV AFPETPPPSY ETVMAAAPPY VPPRYLGPTE GRNSIRYSEL SPLYDTTRVY
     LVDNKSSDIA SLNYQNDHSN FLTTVVQNND YSPIEAGTQT INFDERSRWG GDLKTILHTN
     MPNVNDFMFT TKFKARVMVA RKTNNEGQTI LEYEWAEFVL PEGNYSETMT IDLMNNAIIE
     HYLRVGRQHG VLESDIGVKF DTRNFRLGWD PETQLVTPGV YTNEAFHPDI VLLPGCGVDF
     TESRLSNILG IRKRQPFQEG FVIMYEHLEG GNIPALLDVK KYENSLQDQN TVRGDNFIAL
     NKAARIEPVE TDPKGRSYNL LPDKKNTKYR SWYLAYNYGD PEKGVRSWTL LTTPDVTGGS
     EQVYWSLPDM MQDPVTFRSS RQVSNYPVVA AELLPVHAKS FYNEQAVYSQ LIRQSTALTR
     VFNRFPENQI LVRPPAATIT TVSENVPALT DHGTLPLRSS ISGVQRVTIT DARRRTCPYV
     YKALGIVSPR VLSSRTF