ID   Y1954_ARCFU             Reviewed;         453 AA.
AC   O28325;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Putative amidase AF_1954;
DE            EC=3.5.1.4;
GN   OrderedLocusNames=AF_1954;
OS   Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS   100126 / VC-16).
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC   Archaeoglobus.
OX   NCBI_TaxID=224325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX   PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA   Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA   Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA   Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA   Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA   Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA   Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA   Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA   Smith H.O., Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT   archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; AE000782; AAB89301.1; -; Genomic_DNA.
DR   PIR; A69494; A69494.
DR   RefSeq; WP_010879446.1; NC_000917.1.
DR   AlphaFoldDB; O28325; -.
DR   SMR; O28325; -.
DR   STRING; 224325.AF_1954; -.
DR   EnsemblBacteria; AAB89301; AAB89301; AF_1954.
DR   GeneID; 1485175; -.
DR   KEGG; afu:AF_1954; -.
DR   eggNOG; arCOG01717; Archaea.
DR   HOGENOM; CLU_009600_0_4_2; -.
DR   OMA; DRSDWYR; -.
DR   OrthoDB; 12800at2157; -.
DR   PhylomeDB; O28325; -.
DR   Proteomes; UP000002199; Chromosome.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..453
FT                   /note="Putative amidase AF_1954"
FT                   /id="PRO_0000105239"
FT   ACT_SITE        67
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        142
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        166
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   453 AA;  50063 MW;  72E8D6A2A535F483 CRC64;
     MERAVDIVEK LKGGEIKPAE LVEECLEKIE RLNPKINAFV TLNEKAIEEA KKADVSTPLA
     GLPIAIKDNV ETRGIRTTYC SKFYENYVPE EDAVLVERLK KAGAVIIGKT NMPEFGLIAY
     TDNPMFGPTR NPWDLSRTVG GSSGGSAAAV AAGILPVASG NDGGGSIRIP ASFCGLYGLK
     PSFGRVPCYP SLPIFIGLHS EGFLTRYVED TALMLDLVKG WDIRDVKSLP DEEFSYLKAI
     EEHPDGVRIA FSPDLGYAIV DPEVEEKVRE AAFKLEKFGE VEEVKLSLPN LEQELVTKVV
     LEVVTFIGDR MAEWEKVAFP PYLGFMALAQ SLTFREYIKI EERKMELWKA LRGIFEEYDY
     LITPTVACKP FEIGKLGPEE IAGKAITPIG WMPFTYPFNF TGQPAASIPA GFSKEGLPIG
     MQIVGKPYDD VGVLKVSKAF QDVSPWQDRY PEL