ID   Y2004_MYCTU             Reviewed;         498 AA.
AC   P9WLN3; L0T9W8; P0A5F9; Q10852;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Uncharacterized protein Rv2004c;
GN   OrderedLocusNames=Rv2004c; ORFNames=MTCY39.13;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RA   Suarez T., Cardenas C., Murillo L.A., Avila M., Mejia G., Patarroyo M.E.;
RL   Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [3]
RP   INDUCTION BY NITRIC OXIDE (NO) AND BY HYPOXIA, AND DORMANCY REGULON.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=12953092; DOI=10.1084/jem.20030205;
RA   Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M.,
RA   Sherman D.R., Schoolnik G.K.;
RT   "Inhibition of respiration by nitric oxide induces a Mycobacterium
RT   tuberculosis dormancy program.";
RL   J. Exp. Med. 198:705-713(2003).
RN   [4]
RP   BINDING TO HUMAN CELL LINES, INDUCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=16199660; DOI=10.1110/ps.051592505;
RA   Forero M., Puentes A., Cortes J., Castillo F., Vera R., Rodriguez L.E.,
RA   Valbuena J., Ocampo M., Curtidor H., Rosas J., Garcia J., Barrera G.,
RA   Alfonso R., Patarroyo M.A., Patarroyo M.E.;
RT   "Identifying putative Mycobacterium tuberculosis Rv2004c protein sequences
RT   that bind specifically to U937 macrophages and A549 epithelial cells.";
RL   Protein Sci. 14:2767-2780(2005).
RN   [5]
RP   INDUCTION BY CARBON MONOXIDE (CO).
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=18474359; DOI=10.1016/j.chom.2008.03.007;
RA   Shiloh M.U., Manzanillo P., Cox J.S.;
RT   "Mycobacterium tuberculosis senses host-derived carbon monoxide during
RT   macrophage infection.";
RL   Cell Host Microbe 3:323-330(2008).
RN   [6]
RP   INDUCTION BY CARBON MONOXIDE (CO), AND DORMANCY REGULON.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=18400743; DOI=10.1074/jbc.m802274200;
RA   Kumar A., Deshane J.S., Crossman D.K., Bolisetty S., Yan B.S., Kramnik I.,
RA   Agarwal A., Steyn A.J.;
RT   "Heme oxygenase-1-derived carbon monoxide induces the Mycobacterium
RT   tuberculosis dormancy regulon.";
RL   J. Biol. Chem. 283:18032-18039(2008).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Some isolated peptides of this protein are able to bind to
CC       human U937 monocytoblastic and A549 epithelial cell lines.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC       {ECO:0000269|PubMed:16199660}.
CC   -!- INDUCTION: Expressed in growing cells (at protein level). A member of
CC       the dormancy regulon. Induced in response to reduced oxygen tension
CC       (hypoxia), low levels of nitric oxide (NO) and carbon monoxide (CO). It
CC       is hoped that this regulon will give insight into the latent, or
CC       dormant phase of infection. {ECO:0000269|PubMed:12953092,
CC       ECO:0000269|PubMed:16199660, ECO:0000269|PubMed:18400743,
CC       ECO:0000269|PubMed:18474359}.
CC   -!- MISCELLANEOUS: Has been detected by antibody binding and Western blot
CC       in the cell wall fraction but no signal sequence is predicted by
CC       bioinformatic programs.
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DR   EMBL; U62548; AAB61716.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP44776.1; -; Genomic_DNA.
DR   PIR; B70759; B70759.
DR   RefSeq; NP_216520.1; NC_000962.3.
DR   RefSeq; WP_003410057.1; NZ_NVQJ01000043.1.
DR   AlphaFoldDB; P9WLN3; -.
DR   STRING; 83332.Rv2004c; -.
DR   PaxDb; P9WLN3; -.
DR   DNASU; 888817; -.
DR   GeneID; 888817; -.
DR   KEGG; mtu:Rv2004c; -.
DR   TubercuList; Rv2004c; -.
DR   eggNOG; COG0645; Bacteria.
DR   eggNOG; COG2187; Bacteria.
DR   OMA; FRFVDVM; -.
DR   PhylomeDB; P9WLN3; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046812; F:host cell surface binding; IDA:MTBBASE.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell wall; Nucleotide-binding; Reference proteome; Secreted.
FT   CHAIN           1..498
FT                   /note="Uncharacterized protein Rv2004c"
FT                   /id="PRO_0000103932"
FT   BINDING         329..336
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   498 AA;  54423 MW;  8FCE024F4004501E CRC64;
     MDSPTNDGTC DAHPVTDEPF IDVRETHTAV VVLAGDRAFK AKKPVVTDFC DFRTAEQRER
     ACIREFELNS RLAAQSYLGI AHLSDPSGGH AEPVVVMRRY RDKQRLASMV TAGLPVEGAL
     DAIAEVLARF HQRAQRNRCI DTQGEVGAVA RRWHENLAEL RHHADKVVSG DVIRRIEHMV
     DEFVSGREVL FAGRIKEGCI VDGHADLLAD DIFLVDGEPA LLDCLEFEDE LRYLDRIDDA
     AFLAMDLEFL GRKDLGDYFL AGYAVRSGDT APASLRDFYI AYRAVVRAKV ECVRFSQGKP
     EAAADAVRHL IIATQHLQHA TVRLALVGGN PGTGKSTLAR GVAELVGAQV ISTDDVRRRL
     RDCGVITGEP GVLDSGLYSR ANVVAVYQEA LRKARLLLGS GHSVILDGTW GDPQMRACAR
     RLAADTHSAI VEFRCSATVD VMADRIVARA GGNSDATAEI AAALAARQAD WDTGHRIDTA
     GPRERSVGQA YHIWRSAI