Y2060_ARATH
ID Y2060_ARATH Reviewed; 809 AA.
AC Q8LEV3; C0Z234; O04334; O04335; Q56YN6;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=BTB/POZ domain-containing protein At2g30600;
GN OrderedLocusNames=At2g30600/At2g30610; ORFNames=T6B20.5/T6B20.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP DOMAIN BTB.
RX PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA Vierstra R.D.;
RT "Cullins 3a and 3b assemble with members of the broad
RT complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL J. Biol. Chem. 280:18810-18821(2005).
CC -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LEV3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LEV3-2; Sequence=VSP_040843, VSP_040844;
CC -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB63076.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At2g30600 and At2g30610.; Evidence={ECO:0000305};
CC Sequence=AAB63077.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At2g30600 and At2g30610.; Evidence={ECO:0000305};
CC Sequence=BAD93995.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U93215; AAB63076.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U93215; AAB63077.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08413.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08414.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08415.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08416.1; -; Genomic_DNA.
DR EMBL; AK175155; BAD42918.1; -; mRNA.
DR EMBL; AK176077; BAD43840.1; -; mRNA.
DR EMBL; AK176544; BAD44307.1; -; mRNA.
DR EMBL; AK221285; BAD93995.1; ALT_FRAME; mRNA.
DR EMBL; AK318648; BAH56763.1; -; mRNA.
DR EMBL; AY085216; AAM62449.1; -; mRNA.
DR PIR; D84710; D84710.
DR PIR; E84710; E84710.
DR RefSeq; NP_001031447.1; NM_001036370.2. [Q8LEV3-1]
DR RefSeq; NP_001031448.1; NM_001036371.2. [Q8LEV3-1]
DR RefSeq; NP_180618.2; NM_128612.3. [Q8LEV3-1]
DR RefSeq; NP_850151.1; NM_179820.2. [Q8LEV3-1]
DR AlphaFoldDB; Q8LEV3; -.
DR SMR; Q8LEV3; -.
DR STRING; 3702.AT2G30600.5; -.
DR iPTMnet; Q8LEV3; -.
DR EnsemblPlants; AT2G30600.1; AT2G30600.1; AT2G30600. [Q8LEV3-1]
DR EnsemblPlants; AT2G30600.2; AT2G30600.2; AT2G30600. [Q8LEV3-1]
DR EnsemblPlants; AT2G30600.3; AT2G30600.3; AT2G30600. [Q8LEV3-1]
DR EnsemblPlants; AT2G30600.4; AT2G30600.4; AT2G30600. [Q8LEV3-1]
DR GeneID; 817610; -.
DR Gramene; AT2G30600.1; AT2G30600.1; AT2G30600. [Q8LEV3-1]
DR Gramene; AT2G30600.2; AT2G30600.2; AT2G30600. [Q8LEV3-1]
DR Gramene; AT2G30600.3; AT2G30600.3; AT2G30600. [Q8LEV3-1]
DR Gramene; AT2G30600.4; AT2G30600.4; AT2G30600. [Q8LEV3-1]
DR KEGG; ath:AT2G30600; -.
DR Araport; AT2G30600; -.
DR eggNOG; KOG4276; Eukaryota.
DR eggNOG; KOG4441; Eukaryota.
DR InParanoid; Q8LEV3; -.
DR PhylomeDB; Q8LEV3; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8LEV3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8LEV3; baseline and differential.
DR Genevisible; Q8LEV3; AT.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.710.10; -; 2.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR000421; FA58C.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR022041; Methyltransf_FA.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 2.
DR Pfam; PF00754; F5_F8_type_C; 1.
DR Pfam; PF12248; Methyltransf_FA; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 2.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF54695; SSF54695; 2.
DR PROSITE; PS50097; BTB; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..809
FT /note="BTB/POZ domain-containing protein At2g30600"
FT /id="PRO_0000406779"
FT DOMAIN 211..273
FT /note="BTB 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 351..420
FT /note="BTB 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 466..537
FT /note="BACK"
FT VAR_SEQ 458..481
FT /note="DSVCSVLQVVSSISSCKLIEEMCK -> GWRSVLEEGFCMLSSPSGFVYLIM
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_040843"
FT VAR_SEQ 482..809
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_040844"
FT CONFLICT 103
FT /note="Q -> R (in Ref. 4; BAH56763)"
FT /evidence="ECO:0000305"
FT CONFLICT 284
FT /note="S -> F (in Ref. 4; BAH56763)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 809 AA; 92175 MW; DF137C233D8AC1F8 CRC64;
MVAAKENKFL TVAPFECAWS DDLKFREAGR GCVAFDAFAH NDVTVVFREN VGTQHYHYKK
DNSPHYIVII GSNRNRRLKI QVDGKSVVDE EASDLCRCSL EFQSYWISIY DGLISIGKGR
YPFQNLVFKW QDPKPNCNVQ YVGLSSWDKH VGYRNVSVFP VTHNHILLWK QVDCREVRGD
ESGDEKVVEE GTGYDYEQWG LGNFLESWQL SDTVFLVGEE EMDVPAHKVI LQASGNFPLR
SSDGDVIQLR GVSYPILHAL LQYIYTGRTQ ILESELAPLR DLSSKFEVMS LVRQCEESID
HFKLSKTAFD SCRKVKLLCP ISHPLSGFMF PSAFPVDVGK LVKLYSTGEY SDIKIYLSDH
SLTFQSHKVI LSLWSVAFAK MFTNGMSESH SSTIYLTDVS PEAFKAMMNF MYSGELNMED
TVNFGTELIH LLFLADRFGV VPLHQECCKM LLECLSEDSV CSVLQVVSSI SSCKLIEEMC
KRKFSMHFDY CTTASLDFVL LDQTTFSDIL ESADLTVTSE EKILNAVLMW CMKAEESHSW
GVIDEMMNYA DPKSLFKERL QSLDDLLPHV RFSLLPYELL KRLENSNLSK EIPVFNRLLK
EAASFLTSGL ISPGNEPISR FQHRRSSFKE LQYIRDGDSN GVLHFVGTSY GSHQWVNPVL
AKKINITSSS PTSRFTDPKA LASKAYAGTS FAGPRMEDGH ISSWWVVDLG EEHQLMCNYY
TFRQDGSRAF TRFWKFQGSM DGKTWTDLRV HEDDQTMCKA GQFASWPITA ANALLPFRFF
RLVLTGPTAD TSTPWNFCIC YLELYGYFR
