ID   Y2146_BRADU             Reviewed;         281 AA.
AC   Q45219;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 2.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Probable short-chain type dehydrogenase/reductase blr2146;
DE            EC=1.-.-.-;
GN   OrderedLocusNames=blr2146;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=16349113; DOI=10.1128/aem.59.12.4136-4142.1993;
RA   Tully R.E., Keister D.L.;
RT   "Cloning and mutagenesis of a cytochrome P-450 locus from Bradyrhizobium
RT   japonicum that is expressed anaerobically and symbiotically.";
RL   Appl. Environ. Microbiol. 59:4136-4142(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=9655913; DOI=10.1016/s0167-4781(98)00069-4;
RA   Tully R.E., van Berkum P., Lovins K.W., Keister D.L.;
RT   "Identification and sequencing of a cytochrome P450 gene cluster from
RT   Bradyrhizobium japonicum.";
RL   Biochim. Biophys. Acta 1398:243-255(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; U12678; AAC28892.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC47411.1; -; Genomic_DNA.
DR   PIR; I40211; I40211.
DR   RefSeq; NP_768786.1; NC_004463.1.
DR   RefSeq; WP_011084940.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q45219; -.
DR   SMR; Q45219; -.
DR   STRING; 224911.27350400; -.
DR   EnsemblBacteria; BAC47411; BAC47411; BAC47411.
DR   GeneID; 64067358; -.
DR   KEGG; bja:blr2146; -.
DR   PATRIC; fig|224911.44.peg.1662; -.
DR   eggNOG; COG1028; Bacteria.
DR   HOGENOM; CLU_010194_1_0_5; -.
DR   InParanoid; Q45219; -.
DR   OMA; ITYNSRA; -.
DR   PhylomeDB; Q45219; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..281
FT                   /note="Probable short-chain type dehydrogenase/reductase
FT                   blr2146"
FT                   /id="PRO_0000054821"
FT   REGION          261..281
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        159
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         10..34
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         146
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        117
FT                   /note="T -> R (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        198..204
FT                   /note="LAKLDAC -> R (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        222..239
FT                   /note="EDVAALVAFLLSDDAAFI -> RTWPRWWRSCSPTMLRSS (in Ref. 1
FT                   and 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   281 AA;  28917 MW;  83857CAF2BC7B054 CRC64;
     MGRFEGKVAV VTGAGAGIGK ACALAIAREG GRVVVADIDG SAAIACTAQI AAEAGHALAL
     AIDIADAQAV AALFETAERH FGGVDLLVNN ASAMHLTPRD RAILELELAV WDQTMATNLR
     GTLLCCRQAI PRMIARGGGA IVNMSSCQGL SGDTALTSYA ASKAAMNMLS SSLATQYGHA
     QIRCNAVAPG LIMTERLLAK LDACMQTHLR RHQLLPRVGR PEDVAALVAF LLSDDAAFIT
     GQVVCIDGGM LAHVPTYADG GNSRAARPAG ETAEADAAPR C