CARAC_PSERE
ID CARAC_PSERE Reviewed; 107 AA.
AC Q8GI16;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Ferredoxin CarAc;
DE AltName: Full=Carbazole 1,9a-dioxygenase, ferredoxin component;
DE Short=CARDO;
GN Name=carAc;
OS Pseudomonas resinovorans.
OG Plasmid pCAR1.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=53412;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND NOMENCLATURE.
RC STRAIN=CA10;
RX PubMed=9244273; DOI=10.1128/jb.179.15.4841-4849.1997;
RA Sato S.I., Ouchiyama N., Kimura T., Nojiri H., Yamane H., Omori T.;
RT "Cloning of genes involved in carbazole degradation of Pseudomonas sp.
RT strain CA10: nucleotide sequences of genes and characterization of meta-
RT cleavage enzymes and hydrolase.";
RL J. Bacteriol. 179:4841-4849(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN THE CARBAZOLE
RP DEGRADATION.
RC STRAIN=CA10;
RX PubMed=9244274; DOI=10.1128/jb.179.15.4850-4858.1997;
RA Sato S.I., Nam J.W., Kasuga K., Nojiri H., Yamane H., Omori T.;
RT "Identification and characterization of genes encoding carbazole 1,9a-
RT dioxygenase in Pseudomonas sp. strain CA10.";
RL J. Bacteriol. 179:4850-4858(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CA10;
RX PubMed=11371531; DOI=10.1128/jb.183.12.3663-3679.2001;
RA Nojiri H., Sekiguchi H., Maeda K., Urata M., Nakai S., Yoshida T., Habe H.,
RA Omori T.;
RT "Genetic characterization and evolutionary implications of a car gene
RT cluster in the carbazole degrader Pseudomonas sp. strain CA10.";
RL J. Bacteriol. 183:3663-3679(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pCAR1;
RX PubMed=12547188; DOI=10.1016/s0022-2836(02)01400-6;
RA Maeda K., Nojiri H., Shintani M., Yoshida T., Habe H., Omori T.;
RT "Complete nucleotide sequence of carbazole/dioxin-degrading plasmid pCAR1
RT in Pseudomonas resinovorans strain CA10 indicates its mosaicity and the
RT presence of large catabolic transposon Tn4676.";
RL J. Mol. Biol. 326:21-33(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CA10; PLASMID=pCAR1;
RX PubMed=15466034; DOI=10.1128/jb.186.20.6815-6823.2004;
RA Urata M., Miyakoshi M., Kai S., Maeda K., Habe H., Omori T., Yamane H.,
RA Nojiri H.;
RT "Transcriptional regulation of the ant operon, encoding two-component
RT anthranilate 1,2-dioxygenase, on the carbazole-degradative plasmid pCAR1 of
RT Pseudomonas resinovorans strain CA10.";
RL J. Bacteriol. 186:6815-6823(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pCAR1;
RX PubMed=16672459; DOI=10.1128/aem.72.5.3206-3216.2006;
RA Shintani M., Yano H., Habe H., Omori T., Yamane H., Tsuda M., Nojiri H.;
RT "Characterization of the replication, maintenance, and transfer features of
RT the IncP-7 plasmid pCAR1, which carries genes involved in carbazole and
RT dioxin degradation.";
RL Appl. Environ. Microbiol. 72:3206-3216(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pCAR1;
RX PubMed=17675379; DOI=10.1128/jb.00684-07;
RA Miyakoshi M., Shintani M., Terabayashi T., Kai S., Yamane H., Nojiri H.;
RT "Transcriptome analysis of Pseudomonas putida KT2440 harboring the
RT completely sequenced IncP-7 plasmid pCAR1.";
RL J. Bacteriol. 189:6849-6860(2007).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pCAR1;
RX PubMed=19376894; DOI=10.1128/aem.02373-08;
RA Takahashi Y., Shintani M., Li L., Yamane H., Nojiri H.;
RT "Carbazole-degradative IncP-7 plasmid pCAR1.2 is structurally unstable in
RT Pseudomonas fluorescens Pf0-1, which accumulates catechol, the intermediate
RT of the carbazole degradation pathway.";
RL Appl. Environ. Microbiol. 75:3920-3929(2009).
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pCAR1;
RX PubMed=19270415; DOI=10.1271/bbb.80665;
RA Takahashi Y., Shintani M., Yamane H., Nojiri H.;
RT "The complete nucleotide sequence of pCAR2: pCAR2 and pCAR1 were
RT structurally identical IncP-7 carbazole degradative plasmids.";
RL Biosci. Biotechnol. Biochem. 73:744-746(2009).
RN [10]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pCAR1;
RX PubMed=19134166; DOI=10.1186/1471-2164-10-12;
RA Miyakoshi M., Nishida H., Shintani M., Yamane H., Nojiri H.;
RT "High-resolution mapping of plasmid transcriptomes in different host
RT bacteria.";
RL BMC Genomics 10:12-12(2009).
RN [11]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pCAR1;
RX PubMed=19930443; DOI=10.1111/j.1462-2920.2009.02110.x;
RA Shintani M., Takahashi Y., Tokumaru H., Kadota K., Hara H., Miyakoshi M.,
RA Naito K., Yamane H., Nishida H., Nojiri H.;
RT "Response of the Pseudomonas host chromosomal transcriptome to carriage of
RT the IncP-7 plasmid pCAR1.";
RL Environ. Microbiol. 12:1413-1426(2010).
RN [12]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pCAR1;
RX PubMed=20639326; DOI=10.1128/jb.00591-10;
RA Yun C.S., Suzuki C., Naito K., Takeda T., Takahashi Y., Sai F.,
RA Terabayashi T., Miyakoshi M., Shintani M., Nishida H., Yamane H.,
RA Nojiri H.;
RT "Pmr, a histone-like protein H1 (H-NS) family protein encoded by the IncP-7
RT plasmid pCAR1, is a key global regulator that alters host function.";
RL J. Bacteriol. 192:4720-4731(2010).
RN [13]
RP PROTEIN SEQUENCE OF 1-15, FUNCTION AS A FERREDOXIN, BIOPHYSICOCHEMICAL
RP PROPERTIES, COFACTOR, AND SUBUNIT.
RC STRAIN=CA10;
RX PubMed=12450807; DOI=10.1128/aem.68.12.5882-5890.2002;
RA Nam J.W., Nojiri H., Noguchi H., Uchimura H., Yoshida T., Habe H.,
RA Yamane H., Omori T.;
RT "Purification and characterization of carbazole 1,9a-dioxygenase, a three-
RT component dioxygenase system of Pseudomonas resinovorans strain CA10.";
RL Appl. Environ. Microbiol. 68:5882-5890(2002).
RN [14]
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR
RP (2FE-2S).
RC STRAIN=CA10;
RX PubMed=15645447; DOI=10.1002/prot.20374;
RA Nam J.W., Noguchi H., Fujimoto Z., Mizuno H., Ashikawa Y., Abo M.,
RA Fushinobu S., Kobashi N., Wakagi T., Iwata K., Yoshida T., Habe H.,
RA Yamane H., Omori T., Nojiri H.;
RT "Crystal structure of the ferredoxin component of carbazole 1,9a-
RT dioxygenase of Pseudomonas resinovorans strain CA10, a novel Rieske non-
RT heme iron oxygenase system.";
RL Proteins 58:779-789(2005).
RN [15]
RP X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR
RP (2FE-2S), AND COFACTOR.
RX PubMed=17161368; DOI=10.1016/j.str.2006.10.004;
RA Ashikawa Y., Fujimoto Z., Noguchi H., Habe H., Omori T., Yamane H.,
RA Nojiri H.;
RT "Electron transfer complex formation between oxygenase and ferredoxin
RT components in Rieske nonheme iron oxygenase system.";
RL Structure 14:1779-1789(2006).
CC -!- FUNCTION: Part of the multicomponent carbazole 1,9a-dioxygenase
CC (CARDO), that converts carbazole (CAR) into 2-aminobiphenyl-2,3-diol.
CC Acts as a mediator in the electron transfer from CarAd to CarAa.
CC {ECO:0000269|PubMed:12450807, ECO:0000269|PubMed:9244274}.
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00628,
CC ECO:0000269|PubMed:12450807, ECO:0000269|PubMed:17161368};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00628, ECO:0000269|PubMed:12450807,
CC ECO:0000269|PubMed:17161368};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is between 7 and 7.5. {ECO:0000269|PubMed:12450807};
CC Temperature dependence:
CC Optimum temperature is 30 degrees Celsius.
CC {ECO:0000269|PubMed:12450807};
CC -!- SUBUNIT: Monomer. Carbazole 1,9a-dioxygenase complex consists of a
CC terminal oxygenase component CarAa, a ferredoxin reductase component
CC CarAd and a ferredoxin component CarAc. {ECO:0000269|PubMed:12450807,
CC ECO:0000269|PubMed:15645447, ECO:0000269|PubMed:17161368}.
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DR EMBL; AB047548; BAB32770.1; -; Genomic_DNA.
DR EMBL; AB088420; BAC41549.1; -; Genomic_DNA.
DR RefSeq; NP_758571.1; NC_004444.1.
DR RefSeq; WP_011077882.1; NC_004444.1.
DR PDB; 1VCK; X-ray; 1.90 A; A=1-107.
DR PDB; 2DE5; X-ray; 1.90 A; D/E/F=1-107.
DR PDB; 2DE6; X-ray; 1.80 A; D/E/F=1-107.
DR PDB; 2DE7; X-ray; 2.00 A; D/E/F=1-107.
DR PDB; 3VMG; X-ray; 1.95 A; D/E/F=1-107.
DR PDB; 3VMH; X-ray; 1.85 A; D/E/F=1-107.
DR PDB; 3VMI; X-ray; 2.00 A; D/E/F=1-107.
DR PDB; 4NB8; X-ray; 2.01 A; D/E/F=1-107.
DR PDB; 4NB9; X-ray; 2.05 A; D/E/F=1-107.
DR PDB; 4NBA; X-ray; 2.10 A; D/E/F=1-107.
DR PDB; 4NBB; X-ray; 2.05 A; D/E/F=1-107.
DR PDB; 4NBC; X-ray; 1.94 A; D/E/F=1-107.
DR PDB; 4NBD; X-ray; 1.95 A; D/E=1-107.
DR PDB; 4NBE; X-ray; 2.10 A; D/E=1-107.
DR PDB; 4NBF; X-ray; 2.00 A; D/E/F=1-107.
DR PDB; 4NBG; X-ray; 1.85 A; D/E/F=1-107.
DR PDB; 4NBH; X-ray; 2.15 A; D/E/F=1-107.
DR PDB; 6LLF; X-ray; 1.93 A; D/E/F=1-107.
DR PDB; 6LLH; X-ray; 1.99 A; D/E/F=1-107.
DR PDB; 7BUH; X-ray; 1.79 A; A=1-107.
DR PDB; 7BUI; X-ray; 2.15 A; D/E=1-107.
DR PDBsum; 1VCK; -.
DR PDBsum; 2DE5; -.
DR PDBsum; 2DE6; -.
DR PDBsum; 2DE7; -.
DR PDBsum; 3VMG; -.
DR PDBsum; 3VMH; -.
DR PDBsum; 3VMI; -.
DR PDBsum; 4NB8; -.
DR PDBsum; 4NB9; -.
DR PDBsum; 4NBA; -.
DR PDBsum; 4NBB; -.
DR PDBsum; 4NBC; -.
DR PDBsum; 4NBD; -.
DR PDBsum; 4NBE; -.
DR PDBsum; 4NBF; -.
DR PDBsum; 4NBG; -.
DR PDBsum; 4NBH; -.
DR PDBsum; 6LLF; -.
DR PDBsum; 6LLH; -.
DR PDBsum; 7BUH; -.
DR PDBsum; 7BUI; -.
DR AlphaFoldDB; Q8GI16; -.
DR SMR; Q8GI16; -.
DR DIP; DIP-29166N; -.
DR IntAct; Q8GI16; 1.
DR DrugBank; DB07301; Carbazole.
DR BRENDA; 1.14.12.22; 7692.
DR EvolutionaryTrace; Q8GI16; -.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IDA:UniProtKB.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0046232; P:carbazole catabolic process; IDA:UniProtKB.
DR Gene3D; 2.102.10.10; -; 1.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR Pfam; PF00355; Rieske; 1.
DR SUPFAM; SSF50022; SSF50022; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 1: Evidence at protein level;
KW 2Fe-2S; 3D-structure; Dioxygenase; Direct protein sequencing; Iron;
KW Iron-sulfur; Metal-binding; NAD; Oxidoreductase; Plasmid.
FT CHAIN 1..107
FT /note="Ferredoxin CarAc"
FT /id="PRO_0000419028"
FT DOMAIN 6..102
FT /note="Rieske"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT BINDING 46
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628,
FT ECO:0000269|PubMed:15645447, ECO:0000269|PubMed:17161368"
FT BINDING 48
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628,
FT ECO:0000269|PubMed:15645447, ECO:0000269|PubMed:17161368"
FT BINDING 65
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628,
FT ECO:0000269|PubMed:15645447, ECO:0000269|PubMed:17161368"
FT BINDING 68
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628,
FT ECO:0000269|PubMed:15645447, ECO:0000269|PubMed:17161368"
FT STRAND 5..10
FT /evidence="ECO:0007829|PDB:7BUH"
FT HELIX 11..13
FT /evidence="ECO:0007829|PDB:7BUH"
FT STRAND 19..24
FT /evidence="ECO:0007829|PDB:7BUH"
FT STRAND 27..35
FT /evidence="ECO:0007829|PDB:7BUH"
FT STRAND 38..45
FT /evidence="ECO:0007829|PDB:7BUH"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:7BUH"
FT HELIX 53..55
FT /evidence="ECO:0007829|PDB:7BUH"
FT STRAND 56..59
FT /evidence="ECO:0007829|PDB:7BUH"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:7BUH"
FT TURN 66..68
FT /evidence="ECO:0007829|PDB:7BUH"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:7BUH"
FT TURN 74..76
FT /evidence="ECO:0007829|PDB:7BUH"
FT STRAND 79..81
FT /evidence="ECO:0007829|PDB:7BUH"
FT STRAND 93..96
FT /evidence="ECO:0007829|PDB:7BUH"
FT STRAND 99..103
FT /evidence="ECO:0007829|PDB:7BUH"
SQ SEQUENCE 107 AA; 11367 MW; 4B60F233E57C19A4 CRC64;
MNQIWLKVCA ASDMQPGTIR RVNRVGAAPL AVYRVGDQFY ATEDTCTHGI ASLSEGTLDG
DVIECPFHGG AFNVCTGMPA SSPCTVPLGV FEVEVKEGEV YVAGEKK
