ID   Y217_HAEIN              Reviewed;         176 AA.
AC   P43965;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Putative REP-associated tyrosine transposase;
GN   OrderedLocusNames=HI_0217;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: Transposase responsible for transposition an insertion
CC       sequence (IS) element. Transposition occurs in 2 main steps, excision
CC       from the donor DNA 'top strand' into a single strand circle and its
CC       subsequent reinsertion into the DNA target. This increases the copy
CC       number of the IS. {ECO:0000250|UniProtKB:Q7DF83}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q7DF83};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q7DF83}.
CC   -!- SIMILARITY: Belongs to the transposase 17 family. RAYT subfamily.
CC       {ECO:0000305}.
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DR   EMBL; L42023; AAC21884.1; -; Genomic_DNA.
DR   PIR; A64004; A64004.
DR   RefSeq; NP_438385.1; NC_000907.1.
DR   RefSeq; WP_005694080.1; NC_000907.1.
DR   AlphaFoldDB; P43965; -.
DR   SMR; P43965; -.
DR   DNASU; 951128; -.
DR   EnsemblBacteria; AAC21884; AAC21884; HI_0217.
DR   KEGG; hin:HI_0217; -.
DR   PATRIC; fig|71421.8.peg.225; -.
DR   eggNOG; COG1943; Bacteria.
DR   HOGENOM; CLU_068226_6_0_6; -.
DR   OMA; GCWFFTV; -.
DR   PhylomeDB; P43965; -.
DR   BioCyc; HINF71421:G1GJ1-227-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:InterPro.
DR   Gene3D; 3.30.70.1290; -; 1.
DR   InterPro; IPR002686; Transposase_17.
DR   InterPro; IPR036515; Transposase_17_sf.
DR   Pfam; PF01797; Y1_Tnp; 1.
DR   SMART; SM01321; Y1_Tnp; 1.
DR   SUPFAM; SSF143422; SSF143422; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Metal-binding; Nuclease; Reference proteome; Transposable element;
KW   Transposition.
FT   CHAIN           1..176
FT                   /note="Putative REP-associated tyrosine transposase"
FT                   /id="PRO_0000168537"
FT   ACT_SITE        148
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF83"
FT   BINDING         59
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF83"
FT   BINDING         61
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF83"
SQ   SEQUENCE   176 AA;  21403 MW;  5B49DED14510CC64 CRC64;
     MSNYRRDFTK GGLYFFTIVL QDRTKSYLTD YINEFRSAYK QTCEHYPFET VAICILPDHI
     HLLMQLPEND DNYAIRIAYL KTQFTRQLPK ECRQFNKNRQ KYRESGIWQR RFWEHLIRDD
     KDLANHLDYI YYNPVKHGYV EVVKDWPYSS FHRDVKAEIY PEDWGGNPDL KIKGDI