ID   Y2204_ARCFU             Reviewed;         412 AA.
AC   O28079;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Uncharacterized protein AF_2204;
GN   OrderedLocusNames=AF_2204;
OS   Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS   100126 / VC-16).
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC   Archaeoglobus.
OX   NCBI_TaxID=224325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX   PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA   Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA   Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA   Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA   Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA   Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA   Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA   Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA   Smith H.O., Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT   archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU01266};
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. Anaerobic
CC       sulfatase-maturating enzyme family. {ECO:0000305}.
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DR   EMBL; AE000782; AAB89048.1; -; Genomic_DNA.
DR   PIR; D69525; D69525.
DR   RefSeq; WP_010879693.1; NC_000917.1.
DR   AlphaFoldDB; O28079; -.
DR   SMR; O28079; -.
DR   STRING; 224325.AF_2204; -.
DR   EnsemblBacteria; AAB89048; AAB89048; AF_2204.
DR   GeneID; 1485433; -.
DR   KEGG; afu:AF_2204; -.
DR   eggNOG; arCOG00945; Archaea.
DR   HOGENOM; CLU_009273_3_0_2; -.
DR   OMA; YPCYRFV; -.
DR   OrthoDB; 18533at2157; -.
DR   PhylomeDB; O28079; -.
DR   Proteomes; UP000002199; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR026423; rSAM_cobopep.
DR   InterPro; IPR023867; Sulphatase_maturase_rSAM.
DR   PANTHER; PTHR43273; PTHR43273; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01384; thioether_bond_formation_requi; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR04163; rSAM_cobopep; 1.
DR   TIGRFAMs; TIGR04085; rSAM_more_4Fe4S; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..412
FT                   /note="Uncharacterized protein AF_2204"
FT                   /id="PRO_0000134465"
FT   DOMAIN          50..264
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         64
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         68
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         71
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
SQ   SEQUENCE   412 AA;  47257 MW;  BCAAE920EB580944 CRC64;
     MLKELEVNGK RFFLAPESNF WAIGEREEVE RFYRERKDAL LEEMQRYRFE VDIRTAYINP
     TESCNRNCPY CYIPAEIRER GTKMSYEKLE EILTVLAENG VERVIFHGAE PLMVKDEIFR
     AMEDFDFKYG IQTNATLLEE EDAELLMKKG ASVGISLDSP YKETNDYLRG KGHYDAVMRA
     LDFFDGYRSL NIIMTVNKHN FQHLPAMVDF LAGKVSVMLA NPVRGTSPGG RELRPPEGFE
     DYYIKAIDRA IENTKSGRRI VIGDFANILL GLVAPTSRVL QCDISPCGGG RRFFAVSADG
     IYPCGEFIGM EEFRANLSAL SDWSSLIEKF EVVRKRTVEQ IEECRNCEFR NLCGAPCPAE
     IYAESGTMLR KSPYCEFYKK LALKAMDVIA RGDVRNVLKL ERMKAIYRVE DF