ID   Y2212_VIBVU             Reviewed;         768 AA.
AC   Q8DAI5;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=UPF0313 protein VV1_2212 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=VV1_2212;
OS   Vibrio vulnificus (strain CMCP6).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=216895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; AE016795; AAO10594.1; -; Genomic_DNA.
DR   RefSeq; WP_011080086.1; NC_004459.3.
DR   AlphaFoldDB; Q8DAI5; -.
DR   EnsemblBacteria; AAO10594; AAO10594; VV1_2212.
DR   KEGG; vvu:VV1_2212; -.
DR   HOGENOM; CLU_018288_2_0_6; -.
DR   OMA; TCMYYTG; -.
DR   Proteomes; UP000002275; Chromosome 1.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; S-adenosyl-L-methionine.
FT   CHAIN           1..768
FT                   /note="UPF0313 protein VV1_2212"
FT                   /id="PRO_0000076400"
FT   DOMAIN          363..640
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          674..768
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        691..705
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        715..768
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         377
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         381
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         384
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   768 AA;  85834 MW;  ACEBB7A479EB4877 CRC64;
     MHSDITPIHT HKKYWAECFG TAPFLPTSRK EMDALGWDSC DIVLVTGDAY VDHPSFGMAI
     IGRLLEAQGF RVGIIAQPQW QDKTDFMSLG KPNLFFGVTS GNMDSMINRY TSDRKLRHDD
     AYTPNNEGGK RPDRATLVYS QRCREAYKDV PIVLGGIEAS LRRVAHYDYW SDKVRRSVLL
     DAKADILLFG NAERALVEVA HRLAEGEEIA QMTNIRGTAV NLAAEPEGYT IIDSSRIEKP
     RKEAFIPPNP YEVETQCETK SEEPKAQPIT IRPSRHDAAT TAVRLPSFEK LQNDRILYAH
     ASRILHLETN PYSGRALIQR HGNRELWVNQ APIPLTTEEM DYVFGLPYAR VPHPKYGKAK
     IPAYDMIKTS VNIMRGCFGG CSFCSITEHE GRIIQNRSQE SILTELEEIR DKVPGFTGTI
     SDLGGPTANM YRLGCSDPKA EANCRRPSCV FPGICNKLNT DHKHTIDLYR AARQVKGVKK
     VMIASGVRYD LAIESPEYVK ELVTHHVGGY LKIAPEHTEK GPLDLMMKPG MGTYDRFKEM
     FDKYSQEAGK KQYLIPYFIS AHPGTTDEDM LNLALWLKKN NYECDQVQNF YPSPMCNATS
     MYYSETNPLK RVKYKQREDV PVAKGDRQRR LHKALLRYHD PANWPLIREA LITMGKKYLI
     GDKPGCLVPA EDMDARTPAQ RRKSGRHGAN RFATKHTSTQ PGFPGDKANA GSGKKPTRGG
     QSNSAPSRSG SATGGKHPQR SGANTGGKSS GGKNSPRAGG RNQPSRAR