ID   Y221_MYCTO              Reviewed;         469 AA.
AC   P9WKB6; L0T2V6; P96403;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT0231;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT0231;
GN   OrderedLocusNames=MT0231;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK44452.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK44452.1; ALT_INIT; Genomic_DNA.
DR   PIR; C70961; C70961.
DR   RefSeq; WP_003900834.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKB6; -.
DR   SMR; P9WKB6; -.
DR   EnsemblBacteria; AAK44452; AAK44452; MT0231.
DR   KEGG; mtc:MT0231; -.
DR   PATRIC; fig|83331.31.peg.251; -.
DR   HOGENOM; CLU_024186_4_2_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..469
FT                   /note="Putative diacyglycerol O-acyltransferase MT0231"
FT                   /id="PRO_0000427682"
FT   ACT_SITE        139
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   469 AA;  51862 MW;  8569D83717BA946B CRC64;
     MKRLSGWDAV LLYSETPNVH MHTLKVAVIE LDSDRQEFGV DAFREVIAGR LHKLEPLGYQ
     LVDVPLKFHH PMWREHCQVD LNYHIRPWRL RAPGGRRELD EAVGEIASTP LNRDHPLWEM
     YFVEGLANHR IAVVAKIHHA LADGVASANM MARGMDLLPG PEVGRYVPDP APTKRQLLSA
     AFIDHLRHLG RIPATIRYTT QGLGRVRRSS RKLSPALTMP FTPPPTFMNH RLTPERRFAT
     ATLALIDVKA TAKLLGATIN DMVLAMSTGA LRTLLLRYDG KAEPLLASVP VSYDFSPERI
     SGNRFTGMLV ALPADSDDPL QRVRVCHENA VSAKESHQLL GPELISRWAA YWPPAGAEAL
     FRWLSERDGQ NKVLNLNISN VPGPRERGRV GAALVTEIYS VGPLTAGSGL NITVWSYVDQ
     LNISVLTDGS TVQDPHEVTA GMIADFIEIR RAAGLSVELT VVESAMAQA