ID   Y2247_MYCBO             Reviewed;         520 AA.
AC   P65822; A0A1R3Y1B5; Q10508; X2BKG1;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Putative hydrolase Mb2247c;
DE            EC=3.4.-.-;
DE   Flags: Precursor;
GN   OrderedLocusNames=BQ2027_MB2247C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S33 family. {ECO:0000305}.
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DR   EMBL; LT708304; SIU00855.1; -; Genomic_DNA.
DR   RefSeq; NP_855896.1; NC_002945.3.
DR   RefSeq; WP_003411484.1; NC_002945.4.
DR   AlphaFoldDB; P65822; -.
DR   SMR; P65822; -.
DR   ESTHER; myctu-ym23; AlphaBeta_hydrolase.
DR   EnsemblBacteria; SIU00855; SIU00855; BQ2027_MB2247C.
DR   PATRIC; fig|233413.5.peg.2464; -.
DR   OMA; NQMAGFQ; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR013595; Pept_S33_TAP-like_C.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   Pfam; PF08386; Abhydrolase_4; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..34
FT                   /evidence="ECO:0000255"
FT   CHAIN           35..520
FT                   /note="Putative hydrolase Mb2247c"
FT                   /id="PRO_0000027328"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          105..403
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        232
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        461
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        488
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   520 AA;  55078 MW;  7D05F1533A4C6B91 CRC64;
     MAAMWRRRPL SSALLSFGLL LGGLPLAAPP LAGATEEPGA GQTPGAPVVA PQQSWNSCRE
     FIADTSEIRT ARCATVSVPV DYDQPGGTQA KLAVIRVPAT GQRFGALLVN PGGPGASAVD
     MVAAMAPAIA DTDILRHFDL VGFDPRGVGH STPALRCRTD AEFDAYRRDP MADYSPAGVT
     HVEQVYRQLA QDCVDRMGFS FLANIGTASV ARDMDMVRQA LGDDQINYLG YSYGTELGTA
     YLERFGTHVR AMVLDGAIDP AVSPIEESIS QMAGFQTAFN DYAADCARSP ACPLGTDSAQ
     WVNRYHALVD PLVQKPGKTS DPRGLSYADA TTGTINALYS PQRWKYLTSG LLGLQRGSDA
     GDLLVLADDY DGRDADGHYS NDQDAFNAVR CVDAPTPADP AAWVAADQRI RQVAPFLSYG
     QFTGSAPRDL CALWPVPATS TPHPAAPAGA GKVVVVSTTH DPATPYQSGV DLARQLGAPL
     ITFDGTQHTA VFDGNQCVDS AVMHYFLDGT LPPTSLRCAP