ID   Y2253_VIBCH             Reviewed;         452 AA.
AC   Q9KPV9;
DT   15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Putative zinc metalloprotease VC_2253;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=VC_2253;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; AE003852; AAF95397.1; -; Genomic_DNA.
DR   PIR; C82099; C82099.
DR   RefSeq; NP_231884.1; NC_002505.1.
DR   RefSeq; WP_000129778.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KPV9; -.
DR   SMR; Q9KPV9; -.
DR   STRING; 243277.VC_2253; -.
DR   DNASU; 2613175; -.
DR   EnsemblBacteria; AAF95397; AAF95397; VC_2253.
DR   GeneID; 57740876; -.
DR   KEGG; vch:VC_2253; -.
DR   PATRIC; fig|243277.26.peg.2149; -.
DR   eggNOG; COG0750; Bacteria.
DR   HOGENOM; CLU_025778_0_2_6; -.
DR   OMA; QYMVGFG; -.
DR   BioCyc; VCHO:VC2253-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 2.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR041489; PDZ_6.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 2.
DR   Pfam; PF17820; PDZ_6; 1.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   SMART; SM00228; PDZ; 2.
DR   SUPFAM; SSF50156; SSF50156; 2.
DR   TIGRFAMs; TIGR00054; TIGR00054; 1.
DR   PROSITE; PS50106; PDZ; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Membrane; Metal-binding;
KW   Metalloprotease; Protease; Reference proteome; Transmembrane;
KW   Transmembrane helix; Zinc.
FT   CHAIN           1..452
FT                   /note="Putative zinc metalloprotease VC_2253"
FT                   /id="PRO_0000088475"
FT   TRANSMEM        98..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        378..400
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        428..447
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          197..292
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   ACT_SITE        23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         22
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         26
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   452 AA;  48982 MW;  9123A816B6EA7D91 CRC64;
     MTDILWNFIA FIIALGILVA VHEFGHFWVA RRCGVKVEKF SIGFGKSIWK RVGHDGTEYS
     ISMIPLGGYV KMLDGRVDDV PAEQQAMAFD KQSLWKRSAI VSAGPIFNFL FAIFAYWLVF
     MIGVPAVKPV IGEVTPYSIA AQAGLEPGME IKAVSGVNTP DWESVNMGLI GHIGDDSMTI
     TVSSAEGVGL NEIKTINLRD WNFDPETESA MGALGFKPFT PEISNQLTNV SAQGAGERAG
     LQVGDTVLQI NGQAVEAWQQ VVNAIQSHPN APIAVMVERA GQQVELTLIP DSRELSQGKV
     IGFAGIAPKV AEWPQNYRFE LQFGVFESLG KAVEKSGQVI DLTVSMLKKL LVGDVGLNNL
     SGPISIAKGA GTTADYGFVY FLGFLALISI NLGIINLVPL PMLDGGHLLF FMIEAVIRRP
     VPEKVQEMGY RIGGAIIFSL MAVAIFNDFT RL