ID   Y2285_MYCTO             Reviewed;         445 AA.
AC   P9WKB4; L0TBU7; P67206; Q50680;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT2343;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT2343;
GN   OrderedLocusNames=MT2343;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK46627.1; -; Genomic_DNA.
DR   PIR; A70732; A70732.
DR   RefSeq; WP_003411704.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKB4; -.
DR   SMR; P9WKB4; -.
DR   EnsemblBacteria; AAK46627; AAK46627; MT2343.
DR   GeneID; 45426264; -.
DR   KEGG; mtc:MT2343; -.
DR   PATRIC; fig|83331.31.peg.2521; -.
DR   HOGENOM; CLU_024186_4_1_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..445
FT                   /note="Putative diacyglycerol O-acyltransferase MT2343"
FT                   /id="PRO_0000427683"
FT   ACT_SITE        135
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   445 AA;  47707 MW;  0AF79D2A7EC103EB CRC64;
     MKLLSPLDQM FARMEAPRTP MHIGAFAVFD LPKGAPRRFI RDLYEAISQL AFLPFPFDSV
     IAGGASMAYW RQVQPDPSYH VRLSALPYPG TGRDLGALVE RLHSTPLDMA KPLWELHLIE
     GLTGRQFAMY FKAHHCAVDG LGGVNLIKSW LTTDPEAPPG SGKPEPFGDD YDLASVLAAA
     TTKRAVEGVS AVSELAGRLS SMVLGANSSV RAALTTPRTP FNTRVNRHRR LAVQVLKLPR
     LKAVAHATDC TVNDVILASV GGACRRYLQE LGDLPTNTLT ASVPVGFERD ADTVNAASGF
     VAPLGTSIED PVARLTTISA STTRGKAELL AMSPNALQHY SVFGLLPIAV GQKTGALGVI
     PPLFNFTVSN VVLSKDPLYL SGAKLDVIVP MSFLCDGYGL NVTLVGYTDK VVLGFLGCRD
     TLPHLQRLAQ YTGAAFEELE TAALP