Y2287_ARATH
ID Y2287_ARATH Reviewed; 300 AA.
AC O81004; Q5XVD7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2002, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=GTP-binding protein At2g22870;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2001;
GN Name=EMB2001; OrderedLocusNames=At2g22870; ORFNames=T20K9.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Xiao Y.-L., Underwood B.A., Moskal W.A. Jr., Torian U., Redman J.C.,
RA Wu H.C., Utterback T., Town C.D.;
RT "Reconstruction of cDNA sequences for hypothetical genes in Arabidopsis
RT thaliana from 5' and 3' RACE products.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. EngB GTPase family. {ECO:0000305}.
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DR EMBL; AC004786; AAC32434.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC07367.1; -; Genomic_DNA.
DR EMBL; AY087277; AAM64830.1; -; mRNA.
DR EMBL; AY735585; AAU44455.1; -; mRNA.
DR EMBL; AY773849; AAV63878.1; -; mRNA.
DR PIR; H84617; H84617.
DR RefSeq; NP_565543.1; NM_127852.4.
DR AlphaFoldDB; O81004; -.
DR SMR; O81004; -.
DR STRING; 3702.AT2G22870.1; -.
DR PaxDb; O81004; -.
DR PRIDE; O81004; -.
DR ProteomicsDB; 242479; -.
DR EnsemblPlants; AT2G22870.1; AT2G22870.1; AT2G22870.
DR GeneID; 816818; -.
DR Gramene; AT2G22870.1; AT2G22870.1; AT2G22870.
DR KEGG; ath:AT2G22870; -.
DR Araport; AT2G22870; -.
DR TAIR; locus:2059221; AT2G22870.
DR eggNOG; KOG2486; Eukaryota.
DR HOGENOM; CLU_033732_0_0_1; -.
DR InParanoid; O81004; -.
DR OMA; RMDWSAF; -.
DR OrthoDB; 573373at2759; -.
DR PhylomeDB; O81004; -.
DR PRO; PR:O81004; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O81004; baseline and differential.
DR Genevisible; O81004; AT.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01876; YihA_EngB; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00321; GTPase_EngB; 1.
DR InterPro; IPR030393; G_ENGB_dom.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR019987; GTP-bd_ribosome_bio_YsxC.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01926; MMR_HSR1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR03598; GTPase_YsxC; 1.
DR PROSITE; PS51706; G_ENGB; 1.
PE 2: Evidence at transcript level;
KW GTP-binding; Magnesium; Metal-binding; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..300
FT /note="GTP-binding protein At2g22870"
FT /id="PRO_0000157815"
FT DOMAIN 119..297
FT /note="EngB-type G"
FT BINDING 127..134
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 134
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 154..158
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 156
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 172..175
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 239..242
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 276..278
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 300 AA; 33692 MW; 80053B3F9F0E8BD9 CRC64;
MVLLLRYRSL TINLTPLIPK SQKFHTLQSF RNPNFISIPK ISASTNNPTT TTNRSISDAT
KFAKSVLFIP PGVEIEELTD DMVLPGSNIV IGPFAGHSQI KEVEFVKSSA RARDCPKDDR
PEIAILGRSN VGKSSLINCL VRKKEVALTS KKPGKTQLIN HFLVNKSWYI VDLPGYGFAK
VSDAAKTDWS AFTKGYFLNR DSLVCVLLLI DASVPPQKID LDCANWLGRN NVPMTFVFTK
CDKMKATKGK RPDENIKAFQ QIIRENFKVH PPWILTSSVS GLGRDELLLH MSQLRNYWDQ
