Y2296_ARATH
ID Y2296_ARATH Reviewed; 494 AA.
AC Q9SJG2;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Probable receptor-like protein kinase At2g42960;
DE EC=2.7.11.1;
GN OrderedLocusNames=At2g42960; ORFNames=F7D19.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR EMBL; AC006580; AAM15294.1; -; Genomic_DNA.
DR EMBL; AC006931; AAD21713.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10192.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62978.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62979.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62981.1; -; Genomic_DNA.
DR PIR; D84860; D84860.
DR RefSeq; NP_001318408.1; NM_001337002.1.
DR RefSeq; NP_001325098.1; NM_001337005.1.
DR RefSeq; NP_001325100.1; NM_001337004.1.
DR RefSeq; NP_181825.1; NM_129858.2.
DR AlphaFoldDB; Q9SJG2; -.
DR SMR; Q9SJG2; -.
DR STRING; 3702.AT2G42960.1; -.
DR iPTMnet; Q9SJG2; -.
DR PaxDb; Q9SJG2; -.
DR PRIDE; Q9SJG2; -.
DR ProteomicsDB; 242843; -.
DR EnsemblPlants; AT2G42960.1; AT2G42960.1; AT2G42960.
DR EnsemblPlants; AT2G42960.3; AT2G42960.3; AT2G42960.
DR EnsemblPlants; AT2G42960.4; AT2G42960.4; AT2G42960.
DR EnsemblPlants; AT2G42960.5; AT2G42960.5; AT2G42960.
DR GeneID; 818898; -.
DR Gramene; AT2G42960.1; AT2G42960.1; AT2G42960.
DR Gramene; AT2G42960.3; AT2G42960.3; AT2G42960.
DR Gramene; AT2G42960.4; AT2G42960.4; AT2G42960.
DR Gramene; AT2G42960.5; AT2G42960.5; AT2G42960.
DR KEGG; ath:AT2G42960; -.
DR Araport; AT2G42960; -.
DR TAIR; locus:2045620; AT2G42960.
DR eggNOG; KOG1187; Eukaryota.
DR HOGENOM; CLU_000288_4_1_1; -.
DR InParanoid; Q9SJG2; -.
DR OMA; EGHEPET; -.
DR OrthoDB; 684563at2759; -.
DR PhylomeDB; Q9SJG2; -.
DR PRO; PR:Q9SJG2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJG2; baseline and differential.
DR Genevisible; Q9SJG2; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 3: Inferred from homology;
KW ATP-binding; Kinase; Membrane; Nucleotide-binding; Phosphoprotein;
KW Receptor; Reference proteome; Serine/threonine-protein kinase; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..494
FT /note="Probable receptor-like protein kinase At2g42960"
FT /id="PRO_0000389474"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 183..462
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 442..494
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 442..478
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 309
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 189..197
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 211
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 172
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 256
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 313
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 343
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 348
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 356
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
SQ SEQUENCE 494 AA; 55188 MW; FD7E16AB0CCA05F6 CRC64;
MPPESSLNAE MSKKISFFGL KGLKLWVWVC LVVGVFIVMI LCILSLWITF RRKSRRSSSK
FPFNQIPHVS KDIRVDRAGF QNPHPESLYI EMNDKSTGKT MMSHLGRTKS SDNDTLSQCS
SVNHHERACS SHSGEEGGFG SAGRQYGGGP VTASPLVGLP EISHLGWGHW FTLRDLELAT
NRFAPVNVLG EGGYGVVYRG KLVNGTEVAV KKLLNNLGQA EKEFRVEVEA IGHVRHKNLV
RLLGYCIEGV HRMLVYEYVN SGNLEQWLHG AMRQHGNLTW EARMKIITGT AQALAYLHEA
IEPKVVHRDI KASNILIDDE FNAKLSDFGL AKLLDSGESH ITTRVMGTFG YVAPEYANTG
LLNEKSDIYS FGVLLLEAIT GRDPVDYGRP ANEVNLVEWL KMMVGTRRAE EVVDPRLEPR
PSKSALKRAL LVSLRCVDPE AEKRPRMSQV ARMLESDEHP FHKERRNKRS KTAGMEIVET
KDESLGPSGS ETKP
