ID   Y2306_MYCBO             Reviewed;         445 AA.
AC   P67207; A0A1R3Y0N8; Q50680; X2BKE0;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase Mb2306;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase Mb2306;
GN   OrderedLocusNames=BQ2027_MB2306;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIU00918.1; -; Genomic_DNA.
DR   RefSeq; NP_855955.1; NC_002945.3.
DR   RefSeq; WP_003411704.1; NC_002945.4.
DR   AlphaFoldDB; P67207; -.
DR   SMR; P67207; -.
DR   EnsemblBacteria; SIU00918; SIU00918; BQ2027_MB2306.
DR   GeneID; 45426264; -.
DR   PATRIC; fig|233413.5.peg.2531; -.
DR   OMA; TPFNTRI; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..445
FT                   /note="Putative diacyglycerol O-acyltransferase Mb2306"
FT                   /id="PRO_0000222911"
FT   ACT_SITE        135
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   445 AA;  47707 MW;  0AF79D2A7EC103EB CRC64;
     MKLLSPLDQM FARMEAPRTP MHIGAFAVFD LPKGAPRRFI RDLYEAISQL AFLPFPFDSV
     IAGGASMAYW RQVQPDPSYH VRLSALPYPG TGRDLGALVE RLHSTPLDMA KPLWELHLIE
     GLTGRQFAMY FKAHHCAVDG LGGVNLIKSW LTTDPEAPPG SGKPEPFGDD YDLASVLAAA
     TTKRAVEGVS AVSELAGRLS SMVLGANSSV RAALTTPRTP FNTRVNRHRR LAVQVLKLPR
     LKAVAHATDC TVNDVILASV GGACRRYLQE LGDLPTNTLT ASVPVGFERD ADTVNAASGF
     VAPLGTSIED PVARLTTISA STTRGKAELL AMSPNALQHY SVFGLLPIAV GQKTGALGVI
     PPLFNFTVSN VVLSKDPLYL SGAKLDVIVP MSFLCDGYGL NVTLVGYTDK VVLGFLGCRD
     TLPHLQRLAQ YTGAAFEELE TAALP