ID   Y2350_MYCS2             Reviewed;         257 AA.
AC   A0QUV5; I7FB40;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Probable S-adenosylmethionine-dependent methyltransferase MSMEG_2350/MSMEI_2290;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=MSMEG_2350, MSMEI_2290; ORFNames=MSMEG2349;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION IN MGLP BIOSYNTHESIS, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17640872; DOI=10.1074/jbc.m702676200;
RA   Stadthagen G., Sambou T., Guerin M., Barilone N., Boudou F.,
RA   Kordulakova J., Charles P., Alzari P.M., Lemassu A., Daffe M., Puzo G.,
RA   Gicquel B., Riviere M., Jackson M.;
RT   "Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in
RT   Mycobacterium tuberculosis.";
RL   J. Biol. Chem. 282:27270-27276(2007).
CC   -!- FUNCTION: Probable S-adenosylmethionine-dependent methyltransferase
CC       required for the 6-O-methylation of the polysaccharide backbone of 6-O-
CC       methylglucosyl lipopolysaccharides (MGLP).
CC       {ECO:0000269|PubMed:17640872}.
CC   -!- DISRUPTION PHENOTYPE: Inactivation of this gene results in a dramatic
CC       reduction in the amounts of 6-O-methylglucosyl lipopolysaccharides
CC       (MGLP) synthesized and in the accumulation of precursors of these
CC       molecules. Cells lacking this gene do not grow at high temperature (42
CC       degrees Celsius) in Sauton's medium, although their growth is
CC       comparable to that of wild-type at 30 and 37 degrees Celsius in this
CC       medium. {ECO:0000269|PubMed:17640872}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFP38760.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000480; ABK72111.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP38760.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_886693.1; NC_008596.1.
DR   AlphaFoldDB; A0QUV5; -.
DR   SMR; A0QUV5; -.
DR   STRING; 246196.MSMEI_2290; -.
DR   EnsemblBacteria; ABK72111; ABK72111; MSMEG_2350.
DR   EnsemblBacteria; AFP38760; AFP38760; MSMEI_2290.
DR   KEGG; msg:MSMEI_2290; -.
DR   KEGG; msm:MSMEG_2350; -.
DR   PATRIC; fig|246196.19.peg.2316; -.
DR   eggNOG; COG2227; Bacteria.
DR   OMA; HEVVYQR; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   1: Evidence at protein level;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..257
FT                   /note="Probable S-adenosylmethionine-dependent
FT                   methyltransferase MSMEG_2350/MSMEI_2290"
FT                   /id="PRO_0000413976"
SQ   SEQUENCE   257 AA;  28182 MW;  7D6FE081881A5E76 CRC64;
     MGDPDNALPS ALPLTGERTI PGLAEENYWF RRHEVVYQRL AHRCAGRDVL EAGCGEGYGA
     DLIADVARRV IGLDYDEATV AHVRARYPRV DIRHGNLAEL PLPDASVDVV VNFQVIEHLW
     DQAQFVSECF RVLRPGGVFL VSTPNRITFS PGRDTPLNPF HTRELNAAEL TELLETAGFE
     VEDTLGVFHG AGLAELDARH GGSIIEAQVQ RAVADAPWDE QLLADVAAVR TDDFDLTPAA
     ERDIDDSLDL VAIAVRP