Y2394_ARATH
ID Y2394_ARATH Reviewed; 453 AA.
AC Q3EBR4; O80859;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Putative receptor-like protein kinase At2g30940;
DE EC=2.7.11.1;
GN OrderedLocusNames=At2g30940; ORFNames=F7F1.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3EBR4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3EBR4-2; Sequence=VSP_040169;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC20728.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004669; AAC20728.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08463.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08464.1; -; Genomic_DNA.
DR PIR; E84714; E84714.
DR RefSeq; NP_180651.2; NM_128647.2. [Q3EBR4-2]
DR RefSeq; NP_973571.2; NM_201842.2. [Q3EBR4-1]
DR AlphaFoldDB; Q3EBR4; -.
DR SMR; Q3EBR4; -.
DR STRING; 3702.AT2G30940.2; -.
DR iPTMnet; Q3EBR4; -.
DR PaxDb; Q3EBR4; -.
DR PRIDE; Q3EBR4; -.
DR ProteomicsDB; 243121; -. [Q3EBR4-1]
DR EnsemblPlants; AT2G30940.1; AT2G30940.1; AT2G30940. [Q3EBR4-2]
DR EnsemblPlants; AT2G30940.2; AT2G30940.2; AT2G30940. [Q3EBR4-1]
DR GeneID; 817645; -.
DR Gramene; AT2G30940.1; AT2G30940.1; AT2G30940. [Q3EBR4-2]
DR Gramene; AT2G30940.2; AT2G30940.2; AT2G30940. [Q3EBR4-1]
DR KEGG; ath:AT2G30940; -.
DR Araport; AT2G30940; -.
DR TAIR; locus:2052791; AT2G30940.
DR eggNOG; KOG1187; Eukaryota.
DR InParanoid; Q3EBR4; -.
DR OMA; YFHEDIE; -.
DR OrthoDB; 684563at2759; -.
DR PhylomeDB; Q3EBR4; -.
DR PRO; PR:Q3EBR4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q3EBR4; baseline and differential.
DR Genevisible; Q3EBR4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR Pfam; PF00069; Pkinase; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 3: Inferred from homology;
KW Alternative splicing; ATP-binding; Cell membrane; Kinase; Membrane;
KW Nucleotide-binding; Phosphoprotein; Reference proteome;
KW Serine/threonine-protein kinase; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..453
FT /note="Putative receptor-like protein kinase At2g30940"
FT /id="PRO_0000401354"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 166..428
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 293
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 172..180
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 194
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 155
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 240
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 297
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 322
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT VAR_SEQ 360..361
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040169"
SQ SEQUENCE 453 AA; 51829 MW; 140B2857B02D219E CRC64;
MQEWLEILLI HTLIDSSSLS PQRLRVMNRI ISSQGSDLIK QKLSQHTSFF GIKLWILITA
SASIAFLLVL IISVLLCFIF HRRRCSQEPF RLRSKLCLPL SHIPLTNKQQ IPYNRCGDDI
ESQRISQVGW SSARLSYYTR SFSSTGSFGS FNVFTFMEIK NVTDSFADDN VITKGDSSTV
YRGILMGTVT VAVKRFLPSN SRYEDKDFIT KAEMIANVRH KNVVRLLGYC IEGDERVLVY
EYAEKGDLHE WLHGSAGRNR PLTWRKRMKI IQGVAKGLAY IHEDIEPKIT HQDIRPSKIL
LDYQWNPKIL DVGFIGHSDI PTLIPSPGNM DEKIDVYSFG NMIMELVSGR VSVDQSSPHV
RVYLVDWIKE MVANHMIVDV LDPSLPEFPT IKELKRIVLI SLRCVDPELK ERPKMGDVIH
MLQPHDLLLN NNAIQKAQKI TRSHEVSAIS IRQ
