CARA_ORYSJ
ID CARA_ORYSJ Reviewed; 446 AA.
AC Q6YV23; Q6YV24; Q8S3R8;
DT 24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2013, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Carbamoyl-phosphate synthase small chain, chloroplastic;
DE EC=6.3.5.5;
DE AltName: Full=Carbamoyl-phosphate synthetase glutamine chain;
DE Flags: Precursor;
GN Name=CARA; OrderedLocusNames=Os02g0708100, LOC_Os02g47850;
GN ORFNames=OSJNBb0060O16.25;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15232225;
RA Park Y.-J., Dixit A., Yoo J.-W., Bennetzen J.;
RT "Further evidence of microcolinearity between barley and rice genomes at
RT two orthologous regions.";
RL Mol. Cells 17:492-502(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Involved in arginine biosynthesis. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP +
CC carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate;
CC Xref=Rhea:RHEA:18633, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:58359,
CC ChEBI:CHEBI:456216; EC=6.3.5.5;
CC -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl
CC phosphate from bicarbonate: step 1/1.
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC (S)-dihydroorotate from bicarbonate: step 1/3.
CC -!- SUBUNIT: Composed of two chains; the small (or glutamine) chain
CC promotes the hydrolysis of glutamine to ammonia, which is used by the
CC large (or ammonia) chain to synthesize carbamoyl phosphate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CarA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL87160.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD08105.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD08106.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF09794.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAG95366.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AF480496; AAL87160.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005844; BAD08105.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005844; BAD08106.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008208; BAF09794.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK102055; BAG95366.1; ALT_SEQ; mRNA.
DR RefSeq; XP_015625687.1; XM_015770201.1.
DR RefSeq; XP_015625689.1; XM_015770203.1.
DR AlphaFoldDB; Q6YV23; -.
DR SMR; Q6YV23; -.
DR STRING; 4530.OS02T0708100-02; -.
DR MEROPS; C26.A04; -.
DR PRIDE; Q6YV23; -.
DR GeneID; 4330474; -.
DR KEGG; osa:4330474; -.
DR eggNOG; KOG0370; Eukaryota.
DR HOGENOM; CLU_035901_2_1_1; -.
DR InParanoid; Q6YV23; -.
DR OrthoDB; 566537at2759; -.
DR PlantReactome; R-OSA-1119263; Arginine biosynthesis.
DR PlantReactome; R-OSA-1119495; Citrulline biosynthesis.
DR PlantReactome; R-OSA-1119622; Arginine biosynthesis II (acetyl cycle).
DR UniPathway; UPA00068; UER00171.
DR UniPathway; UPA00070; UER00115.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6YV23; OS.
DR GO; GO:0005951; C:carbamoyl-phosphate synthase complex; IBA:GO_Central.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004088; F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006526; P:arginine biosynthetic process; IBA:GO_Central.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IBA:GO_Central.
DR CDD; cd01744; GATase1_CPSase; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.50.30.20; -; 1.
DR HAMAP; MF_01209; CPSase_S_chain; 1.
DR InterPro; IPR006274; CarbamoylP_synth_ssu.
DR InterPro; IPR002474; CarbamoylP_synth_ssu_N.
DR InterPro; IPR036480; CarbP_synth_ssu_N_sf.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR035686; CPSase_GATase1.
DR InterPro; IPR017926; GATASE.
DR Pfam; PF00988; CPSase_sm_chain; 1.
DR Pfam; PF00117; GATase; 1.
DR SMART; SM01097; CPSase_sm_chain; 1.
DR SUPFAM; SSF52021; SSF52021; 1.
DR SUPFAM; SSF52317; SSF52317; 1.
DR TIGRFAMs; TIGR01368; CPSaseIIsmall; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 2: Evidence at transcript level;
KW Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Chloroplast;
KW Glutamine amidotransferase; Ligase; Nucleotide-binding; Plastid;
KW Pyrimidine biosynthesis; Reference proteome; Transit peptide.
FT TRANSIT 1..50
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 51..446
FT /note="Carbamoyl-phosphate synthase small chain,
FT chloroplastic"
FT /id="PRO_0000423075"
FT DOMAIN 261..446
FT /note="Glutamine amidotransferase type-1"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 336
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 420
FT /evidence="ECO:0000250"
FT ACT_SITE 422
FT /evidence="ECO:0000250"
SQ SEQUENCE 446 AA; 48436 MW; E529103D9D4D3A47 CRC64;
MAAPPATASA PSLRPSAASP RAAAARSVAV PSGPRTVGPR RDGGRFLGVR AAKAVSGVQS
GTVVDDGVQR PWKLSDARLV LEDGSVWKAK SFGASGTQVG EVVFNTSLTG YQEILTDPSY
AGQFVLMTNP HIGNTGVNPD DEESNRCFLA GLIIRNLSIC TSNWRCTETL EEYLMKRNIM
GIYDVDTRAI TRRLREDGSL IGVLSTDQSR TDDELLEMAK NWKIVGVDLI SGVTCDAPYE
WSDKTDSEWE FKKGQSTESF HVVAYDFGIK HNILRRLTSY GCKITVVPAN WPASEVLNLK
PDGVFFSNGP GDPAAVPYAV KTVQEIIGKV PVFGICMGHQ LIGQALGGKT FKMKFGHHGG
NHPVCDLRSG RVDISAQNHN YAVDPESLPE GVKVTHINLN DNSCAGLQYP KMKLLSLQYH
PESSPGPHDS DLAFGEFIEM MKNNRL
