ID   Y242_STRR6              Reviewed;         419 AA.
AC   Q8DRB1;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Putative zinc metalloprotease spr0242;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=spr0242;
OS   Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=171101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-255 / R6;
RX   PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA   Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA   DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA   Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA   Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA   McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA   Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA   Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA   Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT   "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL   J. Bacteriol. 183:5709-5717(2001).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; AE007317; AAK99046.1; -; Genomic_DNA.
DR   PIR; B97902; B97902.
DR   RefSeq; NP_357836.1; NC_003098.1.
DR   RefSeq; WP_000900660.1; NC_003098.1.
DR   AlphaFoldDB; Q8DRB1; -.
DR   SMR; Q8DRB1; -.
DR   STRING; 171101.spr0242; -.
DR   EnsemblBacteria; AAK99046; AAK99046; spr0242.
DR   GeneID; 60233291; -.
DR   GeneID; 66805472; -.
DR   KEGG; spr:spr0242; -.
DR   PATRIC; fig|171101.6.peg.276; -.
DR   eggNOG; COG0750; Bacteria.
DR   HOGENOM; CLU_025778_1_3_9; -.
DR   OMA; QYMVGFG; -.
DR   Proteomes; UP000000586; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 1.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   TIGRFAMs; TIGR00054; TIGR00054; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Metal-binding; Metalloprotease;
KW   Protease; Reference proteome; Transmembrane; Transmembrane helix; Zinc.
FT   CHAIN           1..419
FT                   /note="Putative zinc metalloprotease spr0242"
FT                   /id="PRO_0000088467"
FT   TRANSMEM        169..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        345..367
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        388..410
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         18
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         22
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   419 AA;  45865 MW;  27D7817DDCEF295B CRC64;
     MLGILTFILV FGIIVVVHEF GHFYFAKKSG ILVREFAIGM GPKIFAHIGK DGTAYTIRIL
     PLGGYVRMAG WGDDTTEIKT GTPVSLTLAD DGKVKRINLS GKKLDQTALP MQVTQFDFED
     KLFIKGLVLE EEKTFAVDHD ATVVEADGTE VRIAPLDVQY QNATIWGKLI TNFAGPMNNF
     ILGVVVFWVL IFMQGGVRDV DTNQFHIMPQ GALAKVGVPE TAQITKIGSH EVSNWESLIQ
     AVETETKDKT APTLDVTISE KGSDKQVTVT PEDSQGRYLL GVQPGVKSDF LSMFVGGFTT
     AADSALRILS ALKNLIFQPD LNKLGGPVAI FKASSDAAKN GIENILYFLA MISINIGIFN
     LIPIPALDGG KIVLNILEAI RRKPLKQEIE TYVTLAGVVI MVVLMIAVTW NDIMRLFFR