Y2433_ARCFU
ID Y2433_ARCFU Reviewed; 132 AA.
AC O30238;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 07-APR-2021, sequence version 2.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Putative RNase AF_2433;
DE EC=3.1.-.- {ECO:0000250|UniProtKB:Q8ECH6};
DE AltName: Full=Putative toxin AF_2433;
GN OrderedLocusNames=AF_2433;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
CC -!- FUNCTION: Probable toxic component of a putative type VII toxin-
CC antitoxin (TA) system, probably an RNase. Probably neutralized by
CC cognate antitoxin AF_2432. Neutralization may be due to AMPylation by
CC AF_2432. {ECO:0000250|UniProtKB:Q8ECH6}.
CC -!- SUBUNIT: Homodimer, probably forms a complex with cognate antitoxin
CC AF_2432. {ECO:0000250|UniProtKB:Q8ECH6}.
CC -!- PTM: Modified by cognate antitoxin AF_2432; probably at least 2
CC successive AMPylation events occur on Tyr-97.
CC {ECO:0000250|UniProtKB:A0A0B0QJR1}.
CC -!- SIMILARITY: Belongs to the HepT RNase toxin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB91230.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE000782; AAB91230.1; ALT_INIT; Genomic_DNA.
DR PIR; B69554; B69554.
DR AlphaFoldDB; O30238; -.
DR SMR; O30238; -.
DR STRING; 224325.AF_2433; -.
DR EnsemblBacteria; AAB91230; AAB91230; AF_2433.
DR KEGG; afu:AF_2433; -.
DR eggNOG; arCOG02109; Archaea.
DR HOGENOM; CLU_152343_0_0_2; -.
DR PhylomeDB; O30238; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0110001; C:toxin-antitoxin complex; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR Gene3D; 1.20.120.580; -; 1.
DR InterPro; IPR008201; HepT-like.
DR InterPro; IPR037038; HepT-like_sf.
DR Pfam; PF01934; DUF86; 1.
PE 3: Inferred from homology;
KW Hydrolase; Nuclease; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Toxin-antitoxin system.
FT CHAIN 1..132
FT /note="Putative RNase AF_2433"
FT /id="PRO_0000158267"
FT MOTIF 90..97
FT /note="RX(4)HXY motif"
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
FT ACT_SITE 90
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
FT ACT_SITE 95
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
FT MOD_RES 97
FT /note="O-di-AMP-tyrosine"
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
SQ SEQUENCE 132 AA; 15298 MW; E34CC1B04654F8DA CRC64;
MRRERYLEKI EYIVEALSEI PERVKTPIEV SGVFYNLLTS IESAMDISAM LVKDLGGRVE
DDYSNVEMLK ELGIIDEELA EGLKKCNGLR NWLVHRYNRV DKELVLSSVE EVKALLLKFI
QRVEDVLEKI EP
