ID   Y2449_MYCTU             Reviewed;         419 AA.
AC   O53176; L0TCK0;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 3.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Putative trans-acting enoyl reductase Rv2449c;
DE            EC=1.3.1.-;
GN   OrderedLocusNames=Rv2449c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   PUPYLATION AT LYS-127, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20066036; DOI=10.1371/journal.pone.0008589;
RA   Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E.,
RA   Gygi S.P., Darwin K.H.;
RT   "Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium
RT   tuberculosis.";
RL   PLoS ONE 5:E8589-E8589(2010).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the saccharopine dehydrogenase family. Enoyl
CC       reductase subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP45242.1; -; Genomic_DNA.
DR   PIR; H70863; H70863.
DR   RefSeq; NP_216965.1; NC_000962.3.
DR   RefSeq; WP_003412609.1; NZ_NVQJ01000024.1.
DR   AlphaFoldDB; O53176; -.
DR   STRING; 83332.Rv2449c; -.
DR   PaxDb; O53176; -.
DR   DNASU; 885894; -.
DR   GeneID; 885894; -.
DR   KEGG; mtu:Rv2449c; -.
DR   TubercuList; Rv2449c; -.
DR   eggNOG; COG3268; Bacteria.
DR   InParanoid; O53176; -.
DR   OMA; YVARMEQ; -.
DR   PhylomeDB; O53176; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009247; P:glycolipid biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005097; Sacchrp_dh_NADP.
DR   Pfam; PF03435; Sacchrp_dh_NADP; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Isopeptide bond; Membrane; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation.
FT   CHAIN           1..419
FT                   /note="Putative trans-acting enoyl reductase Rv2449c"
FT                   /id="PRO_0000396094"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          197..232
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CROSSLNK        127
FT                   /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT                   with Q-Cter in protein Pup)"
FT                   /evidence="ECO:0000269|PubMed:20066036"
SQ   SEQUENCE   419 AA;  44379 MW;  83DA2104BC7DC630 CRC64;
     MTATPREFDI VLYGATGFVG KLTAEYLARA GGDARIALAG RSTQRVLAVR EALGESAQTW
     PILTADASLP STLQAMAARA QVVVTTVGPY TRYGLPLVAA CAAAGTDYAD LTGEPMFMRN
     SIDLYHKQAA DTGARIVHAC GFDSVPSDLS VYALYHAARE DGAGELTDTN CVVRSFKGGF
     SGGTIASMLE VLSTASNDPD ARRQLSDPYM LSPDRGAEPE LGPQPDLPSR RGRRLAPELA
     GVWTAGFIMA PTNTRIVRRS NALLDWAYGR RFRYSETMSV GSTVLAPVVS VVGGGVGNAM
     FGLASRYIRL LPRGLVKRVV PKPGTGPSAA ARERGYYRIE TYTTTTTGAR YLARMAQDGD
     PGYKATSVLL GECGLALALD RDKLSDMRGV LTPAAAMGDA LLERLPAAGV SLQTTRLAS