ID   Y2484_MYCTO             Reviewed;         491 AA.
AC   P9WKB2; L0TCH6; O53209;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT2557;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT2557;
GN   OrderedLocusNames=MT2557;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK46861.1; -; Genomic_DNA.
DR   PIR; C70868; C70868.
DR   RefSeq; WP_003899345.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKB2; -.
DR   SMR; P9WKB2; -.
DR   EnsemblBacteria; AAK46861; AAK46861; MT2557.
DR   KEGG; mtc:MT2557; -.
DR   PATRIC; fig|83331.31.peg.2758; -.
DR   HOGENOM; CLU_024186_2_0_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..491
FT                   /note="Putative diacyglycerol O-acyltransferase MT2557"
FT                   /id="PRO_0000427684"
FT   ACT_SITE        145
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   491 AA;  52309 MW;  C2ACCE458ADF82DD CRC64;
     MAESGESPRL SDELGPVDYL MHRGEANPRT RSGIMALELL DGTPDWDRFR TRFENASRRV
     LRLRQKVVVP TLPTAAPRWV VDPDFNLDFH VRRVRVSGPA TLREVLDLAE VILQSPLDIS
     RPLWTATLVE GMADGRAAML LHVSHAVTDG VGGVEMFAQI YDLERDPPPR STPPQPIPED
     LSPNDLMRRG INHLPIAVVG GVLDALSGAV SMAGRAVLEP VSTVSGILGY ARSGIRVLNR
     AAEPSPLLRR RSLTTRTEAI DIRLADLHKA AKAGGGSIND AYLAGLCGAL RRYHEALGVP
     ISTLPMAVPV NLRAEGDAAG GNQFTGVNLA APVGTIDPVA RMKKIRAQMT QRRDEPAMNI
     IGSIAPVLSV LPTAVLEGIT GSVIGSDVQA SNVPVYPGDT YLAGAKILRQ YGIGPLPGVA
     MMVVLISRGG WCTVTVRYDR ASVRNDELFA QCLQAGFDEI LALAGGPAPR VLPASFDTQG
     AGSVPRSVSG S