Y2484_MYCTU
ID Y2484_MYCTU Reviewed; 491 AA.
AC P9WKB3; L0TCH6; O53209;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=Putative diacyglycerol O-acyltransferase Rv2484c;
DE EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE AltName: Full=Putative triacylglycerol synthase Rv2484c;
GN OrderedLocusNames=Rv2484c; ORFNames=MTV008.40c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP EXPRESSION IN E.COLI, AND INDUCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=15262939; DOI=10.1128/jb.186.15.5017-5030.2004;
RA Daniel J., Deb C., Dubey V.S., Sirakova T.D., Abomoelak B., Morbidoni H.R.,
RA Kolattukudy P.E.;
RT "Induction of a novel class of diacylglycerol acyltransferases and
RT triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into
RT a dormancy-like state in culture.";
RL J. Bacteriol. 186:5017-5030(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Upon expression in E.coli functions very weakly as a
CC triacylglycerol synthase, making triacylglycerol (TG) from diolein and
CC long-chain fatty acyl-CoA. Has no wax synthase activity.
CC {ECO:0000269|PubMed:15262939}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC -!- INDUCTION: Constitutively expressed at a low level, it is not further
CC induced by hypoxia or nitric oxide exposure.
CC {ECO:0000269|PubMed:15262939}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP45278.1; -; Genomic_DNA.
DR PIR; C70868; C70868.
DR RefSeq; NP_217000.1; NC_000962.3.
DR RefSeq; WP_003899345.1; NZ_NVQJ01000067.1.
DR AlphaFoldDB; P9WKB3; -.
DR SMR; P9WKB3; -.
DR STRING; 83332.Rv2484c; -.
DR PaxDb; P9WKB3; -.
DR PRIDE; P9WKB3; -.
DR DNASU; 888623; -.
DR GeneID; 888623; -.
DR KEGG; mtu:Rv2484c; -.
DR TubercuList; Rv2484c; -.
DR eggNOG; COG1020; Bacteria.
DR OMA; WTATLIE; -.
DR PhylomeDB; P9WKB3; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0001666; P:response to hypoxia; IBA:GO_Central.
DR GO; GO:0071731; P:response to nitric oxide; IBA:GO_Central.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.30.559.10; -; 1.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650; PTHR31650; 1.
DR Pfam; PF03007; WES_acyltransf; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW Reference proteome; Transferase.
FT CHAIN 1..491
FT /note="Putative diacyglycerol O-acyltransferase Rv2484c"
FT /id="PRO_0000222912"
FT ACT_SITE 145
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
SQ SEQUENCE 491 AA; 52309 MW; C2ACCE458ADF82DD CRC64;
MAESGESPRL SDELGPVDYL MHRGEANPRT RSGIMALELL DGTPDWDRFR TRFENASRRV
LRLRQKVVVP TLPTAAPRWV VDPDFNLDFH VRRVRVSGPA TLREVLDLAE VILQSPLDIS
RPLWTATLVE GMADGRAAML LHVSHAVTDG VGGVEMFAQI YDLERDPPPR STPPQPIPED
LSPNDLMRRG INHLPIAVVG GVLDALSGAV SMAGRAVLEP VSTVSGILGY ARSGIRVLNR
AAEPSPLLRR RSLTTRTEAI DIRLADLHKA AKAGGGSIND AYLAGLCGAL RRYHEALGVP
ISTLPMAVPV NLRAEGDAAG GNQFTGVNLA APVGTIDPVA RMKKIRAQMT QRRDEPAMNI
IGSIAPVLSV LPTAVLEGIT GSVIGSDVQA SNVPVYPGDT YLAGAKILRQ YGIGPLPGVA
MMVVLISRGG WCTVTVRYDR ASVRNDELFA QCLQAGFDEI LALAGGPAPR VLPASFDTQG
AGSVPRSVSG S
