ID   Y249_CLOB6              Reviewed;         639 AA.
AC   C3KYN9;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=UPF0313 protein CLJ_B0249 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=CLJ_B0249;
OS   Clostridium botulinum (strain 657 / Type Ba4).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=515621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=657 / Type Ba4;
RA   Shrivastava S., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A.,
RA   Smith T.J., Sutton G., Brettin T.S.;
RT   "Genome sequence of Clostridium botulinum Ba4 strain 657.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; CP001083; ACQ54848.1; -; Genomic_DNA.
DR   RefSeq; WP_012721331.1; NC_012658.1.
DR   AlphaFoldDB; C3KYN9; -.
DR   EnsemblBacteria; ACQ54848; ACQ54848; CLJ_B0249.
DR   KEGG; cbi:CLJ_B0249; -.
DR   HOGENOM; CLU_018288_2_0_9; -.
DR   OMA; YKLEYYE; -.
DR   Proteomes; UP000002333; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; S-adenosyl-L-methionine.
FT   CHAIN           1..639
FT                   /note="UPF0313 protein CLJ_B0249"
FT                   /id="PRO_1000214114"
FT   DOMAIN          295..566
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          597..639
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         309
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         313
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         316
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   639 AA;  73786 MW;  3F432F75F3197B12 CRC64;
     MSNMDFLPIS KEDLKKRNID VLDFIIVTGD AYVDHPSFGT AIIGRVLERE GFTVGIIAQP
     NWNNIEDFKK LGKPKYGFLV NSGNIDSMVN HYTASKKKRH DDFYSPGGKS GYRPDRAVIV
     YCNKIKEAFK DSPIIIGGIE ASLRRFAHYD YWDNSVRRSI LEDSSADLLI YGMGEKPIVQ
     VSNLLRYGMK IDSIKNVRGT TYIERDISPL KDYIEIPSFE EVSTNKKSYA EAYKIQYYEQ
     DSIRGKTLVQ KHKERYVVQN PPQPPLSQEE MDEVYALPYA RTYHPMYEAE GGIPAIKEVK
     FSITSHRGCY GSCSFCALTF HQGRVIQNRS QDSILKEANM MTNMKDFKGY IHDVGGPTAN
     FRHRACKVQE EHGTCKNKQC VFPKACKNLI IDHKEYLSLL KKIRKIPNVK KVFIRSGIRF
     DYLMYDKNDE FFKELCEHHI SGQLKVAPEH ISDKVLNLMG KPTRNVYDSF VKKYYDINKK
     IHKNQFLVPY LMSSHPGSDL KAAIELAQYI KKMGYTPEQV QDFYPTPGSL STTMYYTGIN
     PLTEEKVYVP KEQKEKRMQR SLLQFSIPDN YDLVKEALIK AHREDLIGNG PDCLIPYNKP
     YKKSHKKNNA KNKNNNYNKN KDVSKKNKKN SLSKHKKRK