ID   Y251_CLOBJ              Reviewed;         645 AA.
AC   C1FQT8;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=UPF0313 protein CLM_0251 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=CLM_0251;
OS   Clostridium botulinum (strain Kyoto / Type A2).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=536232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Kyoto / Type A2;
RA   Shrivastava S., Brinkac L.M., Brown J.L., Bruce D., Detter C.C.,
RA   Johnson E.A., Munk C.A., Smith L.A., Smith T.J., Sutton G., Brettin T.S.;
RT   "Genome sequence of Clostridium botulinum A2 Kyoto.";
RL   Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; CP001581; ACO87244.1; -; Genomic_DNA.
DR   RefSeq; WP_011948014.1; NC_012563.1.
DR   AlphaFoldDB; C1FQT8; -.
DR   EnsemblBacteria; ACO87244; ACO87244; CLM_0251.
DR   GeneID; 5187168; -.
DR   KEGG; cby:CLM_0251; -.
DR   eggNOG; COG1032; Bacteria.
DR   HOGENOM; CLU_018288_2_0_9; -.
DR   OMA; YKLEYYE; -.
DR   Proteomes; UP000001374; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; S-adenosyl-L-methionine.
FT   CHAIN           1..645
FT                   /note="UPF0313 protein CLM_0251"
FT                   /id="PRO_1000165087"
FT   DOMAIN          295..566
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          598..645
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         309
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         313
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         316
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   645 AA;  74555 MW;  AA2CFA5147DDFD57 CRC64;
     MSNMDFLPIS KEDLKKRNID VLDFIIVTGD AYVDHPSFGT AIIGRVLERE GFTVGIIAQP
     NWNNIEDFKK LGKPKYGFLV NSGNIDSMVN HYTASKKKRH DDFYSPGGKS GYRPDRAVIV
     YCNKIKEAFK DSPIIIGGIE ASLRRFAHYD YWDNSVRRSI LEDSSADLLI YGMGEKPIVQ
     VSNLLRYGMK IDSIKNVRGT TYIEKDISSL KDYIEIPSFE EVSTNKKSYA EAYKIQYYEQ
     DSIRGKTLVQ KHKERYVVQN PPQPPLSQEE MDEVYALPYA RTYHPMYEAE GGIPAIKEVK
     FSITSHRGCY GSCSFCALTF HQGRVIQNRS QDSILKEANM MTNMKDFKGY IHDVGGPTAN
     FRHRACKVQE KHGTCKNKQC VFPKACKNLI VDHKEYLSLL RKIRKIPNVK KVFIRSGIRF
     DYLMYDKNDE FFKELCEHHI SGQLKVAPEH ISDKVLNLMG KPTRNVYDSF VKKYYDINKK
     IHKNQFLVPY LMSSHPGSDL KAAIELAQYI KKMGYTPEQV QDFYPTPGSL STTMYYTGIN
     PLTEEKVYIP KDQKEKRMQR ALLQFSIHDN YDLVKEALIK AHREDLIGNG PDCLIPYNKP
     YKKSHKKNNA KNNNNHYNKN NNYNKNKDIS KKNKKNSLSK HKKRK