ID   Y254_BACTN              Reviewed;         620 AA.
AC   Q8AB57;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=UPF0313 protein BT_0254 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=BT_0254;
OS   Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 /
OS   CCUG 10774 / NCTC 10582 / VPI-5482 / E50).
OC   Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC   Bacteroides.
OX   NCBI_TaxID=226186;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC   VPI-5482 / E50;
RX   PubMed=12663928; DOI=10.1126/science.1080029;
RA   Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C.,
RA   Hooper L.V., Gordon J.I.;
RT   "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis.";
RL   Science 299:2074-2076(2003).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; AE015928; AAO75361.1; -; Genomic_DNA.
DR   RefSeq; NP_809167.1; NC_004663.1.
DR   RefSeq; WP_011107187.1; NC_004663.1.
DR   AlphaFoldDB; Q8AB57; -.
DR   STRING; 226186.BT_0254; -.
DR   PaxDb; Q8AB57; -.
DR   PRIDE; Q8AB57; -.
DR   EnsemblBacteria; AAO75361; AAO75361; BT_0254.
DR   GeneID; 60926218; -.
DR   KEGG; bth:BT_0254; -.
DR   PATRIC; fig|226186.12.peg.255; -.
DR   eggNOG; COG1032; Bacteria.
DR   HOGENOM; CLU_018288_2_0_10; -.
DR   InParanoid; Q8AB57; -.
DR   OMA; TCMYYTG; -.
DR   Proteomes; UP000001414; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0044260; P:cellular macromolecule metabolic process; IEA:UniProt.
DR   GO; GO:0043412; P:macromolecule modification; IEA:UniProt.
DR   GO; GO:0032259; P:methylation; IEA:InterPro.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:UniProt.
DR   GO; GO:0044238; P:primary metabolic process; IEA:UniProt.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..620
FT                   /note="UPF0313 protein BT_0254"
FT                   /id="PRO_0000076379"
FT   DOMAIN          311..591
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         325
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         329
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         332
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   620 AA;  71197 MW;  77A4F7E9FCAEB00C CRC64;
     MKEYRLTDWL PTTKKEVELR GWDELDVILF SGDAYVDHPS FGAAVIGRIL EAEGLKVAII
     PQPNWRDDLR DFRKLGRPRL FFGISAGSMD SMVNKYTANK RLRSEDAYTP DGRPDMRPEY
     PSIVYSQILK RLYPDVPVIL GSIEASLRRL SHYDYWQDKV QKSILCDSGA DLLIYGMGEK
     PIVELTRKMK ELLPAEDASL TAGELKKIAG TIPQTAYLCR ATEWTPAADD IQLYSHEECL
     ADKKKQASNF RHIEEESNKY AASRITQEVD NKVVVVNPPY PPMSQEELDH SYDLPYTRLP
     HPKYKGKRIP AYDMIKFSVN IHRGCFGGCA FCTISAHQGK FIVSRSKKSI LNEVKEVMQL
     PDFKGYLSDL GGPSANMYQM KGKDEAICKK CKRPSCIHPK VCPNLNSDHC PLLDIYKAVD
     AIPGIKKSFI GSGVRYDLLL HQSKDAATNR STAEYTRELI VNHVSGRLKV APEHTSDRVL
     SIMRKPSFEQ FETFKKIFDR INREENLRQQ LIPYFISSHP GCKEEDMAEL AVITKRLDFH
     LEQVQDFTPT PMTVATEAWY SGFHPYTLEP VFSAKTQREK LAQRQFFFWY KPEERKNILN
     ELRRIGRQDL IDKLYGKRNK