ID   Y2565_MYCTU             Reviewed;         583 AA.
AC   P9WIY7; L0TA62; P0A642; Q50733;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Uncharacterized NTE family protein Rv2565;
GN   OrderedLocusNames=Rv2565; ORFNames=MTCY9C4.03c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- SIMILARITY: Belongs to the NTE family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45361.1; -; Genomic_DNA.
DR   PIR; A70729; A70729.
DR   RefSeq; NP_217081.1; NC_000962.3.
DR   RefSeq; WP_003901427.1; NZ_NVQJ01000023.1.
DR   AlphaFoldDB; P9WIY7; -.
DR   SMR; P9WIY7; -.
DR   STRING; 83332.Rv2565; -.
DR   PaxDb; P9WIY7; -.
DR   DNASU; 887288; -.
DR   GeneID; 887288; -.
DR   KEGG; mtu:Rv2565; -.
DR   TubercuList; Rv2565; -.
DR   eggNOG; COG0664; Bacteria.
DR   eggNOG; COG1752; Bacteria.
DR   OMA; AGEMAMI; -.
DR   PhylomeDB; P9WIY7; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0004622; F:lysophospholipase activity; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0046470; P:phosphatidylcholine metabolic process; IEA:InterPro.
DR   CDD; cd00038; CAP_ED; 1.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR001423; LysoPLipase_patatin_CS.
DR   InterPro; IPR002641; PNPLA_dom.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   Pfam; PF00027; cNMP_binding; 1.
DR   Pfam; PF01734; Patatin; 1.
DR   SMART; SM00100; cNMP; 1.
DR   SUPFAM; SSF51206; SSF51206; 1.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
DR   PROSITE; PS51635; PNPLA; 1.
DR   PROSITE; PS01237; UPF0028; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Reference proteome.
FT   CHAIN           1..583
FT                   /note="Uncharacterized NTE family protein Rv2565"
FT                   /id="PRO_0000172536"
FT   DOMAIN          309..469
FT                   /note="PNPLA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           313..318
FT                   /note="GXGXXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           340..344
FT                   /note="GXSXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           456..458
FT                   /note="DGA/G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        342
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        456
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   BINDING         24..140
FT                   /ligand="a nucleoside 3',5'-cyclic phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58464"
SQ   SEQUENCE   583 AA;  62124 MW;  EC6EE062BAEC62F7 CRC64;
     MTTARRRPKR RGTDARTALR NVPILADIDD EQLERLATTV ERRHVPANQW LFHAGEPADS
     IYIVDSGRFV AVAPEGHVFA EMASGDSIGD LGVIAGAARS AGVRALRDGV VWRIAAETFT
     DMLEATPLLQ SAMLRAMARM LRQSRPAKTA RRPRVIGVVS NGDTAAAPMV DAIATSLDSH
     GRTAVIAPPV ETTSAVQEYD ELVEAFSETL DRAERSNDWV LVVADRGAGD LWRHYVSAQS
     DRLVVLVDQR YPPDAVDSLA TQRPVHLITC LAEPDPSWWD RLAPVSHHPA NSDGFGALAR
     RIAGRSLGLV MAGGGARGLA HFGVYQELTE AGVVIDRFGG TSSGAIASAA FALGMDAGDA
     IAAAREFIAG SDPLGDYTIP ISALTRGGRV DRLVQGFFGN TLIEHLPRGF FSVSADMITG
     DQIIHRRGSV SGAVRASISI PGLIPPVHNG EQLLVDGGLL NNLPANVMCA DTDGEVICVD
     LRRTFVPSKG FGLLPPIVTP PGLLRRLLTG TDNALPPLQE TLLRAFDLAA STANLRELPR
     VAAIIEPDVS KIGVLNFKQI DAALEAGRMA ARAALQAQPD LVR