ID   Y2578_MYCTU             Reviewed;         340 AA.
AC   P9WL79; L0TBP0; P65023; Q50643;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=Uncharacterized protein Rv2578c;
GN   OrderedLocusNames=Rv2578c; ORFNames=MTCY227.23;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP45374.1; -; Genomic_DNA.
DR   PIR; A70725; A70725.
DR   RefSeq; NP_217094.1; NC_000962.3.
DR   RefSeq; WP_003413359.1; NZ_NVQJ01000023.1.
DR   AlphaFoldDB; P9WL79; -.
DR   STRING; 83332.Rv2578c; -.
DR   PaxDb; P9WL79; -.
DR   DNASU; 887427; -.
DR   GeneID; 45426580; -.
DR   GeneID; 887427; -.
DR   KEGG; mtu:Rv2578c; -.
DR   TubercuList; Rv2578c; -.
DR   eggNOG; COG1533; Bacteria.
DR   OMA; GNTDCYQ; -.
DR   PhylomeDB; P9WL79; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR040086; MJ0683-like.
DR   InterPro; IPR007197; rSAM.
DR   PANTHER; PTHR43432; PTHR43432; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01084; Uncharacterised_Radical_SAM_Su; 1.
DR   SMART; SM00729; Elp3; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S; Cell membrane; Iron; Iron-sulfur; Membrane; Metal-binding;
KW   Reference proteome; S-adenosyl-L-methionine; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..340
FT                   /note="Uncharacterized protein Rv2578c"
FT                   /id="PRO_0000104061"
FT   TRANSMEM        140..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          58..307
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         72
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         76
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         79
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   340 AA;  37176 MW;  504623D48273E2D9 CRC64;
     MRWARQAVAV NGMPVDDGAL PGLQRIGLVR SVRAPQFDGI TFHEVLCKSA LNKVPNAAAL
     PFRYTVNGYR GCSHACRYCF ARPTHEYLDF NPGTDFDTQV VVKTNVAAVL RHELRRPSWR
     RETVALGTNT DPYQRAEGRY ALMPGIIGAL AASGTPLSIL TKGTLLRRDL PLIAEAAQQV
     PVSVAVSLAV GDPELHRDVE SGTPTPQARL ALITAIRAAG LDCHVMVAPV LPQLTDSGEH
     LDQLLGQIAA AGATGVTVFG LHLRGSTRGW FMCWLARAHP ELVSRYRELY RRGPYLPPSY
     REMLRERVAP LIAKYRLAGD HRPAPPETEA ALVPVQATLF