ID   Y258_HELPY              Reviewed;         348 AA.
AC   P56136;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=Putative zinc metalloprotease HP_0258;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=HP_0258;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; AE000511; AAD07326.1; -; Genomic_DNA.
DR   PIR; B64552; B64552.
DR   RefSeq; NP_207056.1; NC_000915.1.
DR   RefSeq; WP_001863029.1; NC_018939.1.
DR   AlphaFoldDB; P56136; -.
DR   SMR; P56136; -.
DR   STRING; 85962.C694_01305; -.
DR   PaxDb; P56136; -.
DR   EnsemblBacteria; AAD07326; AAD07326; HP_0258.
DR   KEGG; hpy:HP_0258; -.
DR   PATRIC; fig|85962.47.peg.278; -.
DR   eggNOG; COG0750; Bacteria.
DR   OMA; QYMVGFG; -.
DR   PhylomeDB; P56136; -.
DR   Proteomes; UP000000429; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 1.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   TIGRFAMs; TIGR00054; TIGR00054; 2.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Membrane; Metal-binding;
KW   Metalloprotease; Protease; Reference proteome; Transmembrane;
KW   Transmembrane helix; Zinc.
FT   CHAIN           1..348
FT                   /note="Putative zinc metalloprotease HP_0258"
FT                   /id="PRO_0000088443"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        247..267
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        275..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        324..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          106..175
FT                   /note="PDZ"
FT   ACT_SITE        17
FT                   /evidence="ECO:0000255"
FT   BINDING         16
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255"
FT   BINDING         20
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   348 AA;  38464 MW;  3F849E05B1A92DA0 CRC64;
     MFIVAVLMLA FLIFVHELGH FTIARICGVK VEVFSIGFGK KLCFFKLFGT QFALSLIPLG
     GYVKLKGMDK EENGMNETTD DSYAQKSPFQ KLWILFGGAF FNFLFAILVY FFLALGGEKV
     LLPVIGDLDK NALEAGLLKG DKILSINHKK IASFREIRSV VARARGELVL EIERNHQVLE
     KRLTPKIVAV ISDSNDPNEM IRYKAIGIKP DMQKMGVVSY SLFQAFEKAL SRFKEGVVLI
     VDSLRRLIMG SSSVKELSGV VGIVGALSHA NSLSMLLLFG AFLSINLGIL NLLPIPALDG
     AQMLGVVFKN IFHITLPTPI QNALWLAGVG FLVFIMFLGL FNDLTRLL