ID   Y2594_MYCBO             Reviewed;         583 AA.
AC   P0A643; A0A1R3Y1K7; Q50733; X2BLC0;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Uncharacterized NTE family protein Mb2594;
GN   OrderedLocusNames=BQ2027_MB2594;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- SIMILARITY: Belongs to the NTE family. {ECO:0000305}.
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DR   EMBL; LT708304; SIU01212.1; -; Genomic_DNA.
DR   RefSeq; NP_856240.1; NC_002945.3.
DR   RefSeq; WP_003901427.1; NC_002945.4.
DR   AlphaFoldDB; P0A643; -.
DR   SMR; P0A643; -.
DR   EnsemblBacteria; SIU01212; SIU01212; BQ2027_MB2594.
DR   PATRIC; fig|233413.5.peg.2853; -.
DR   OMA; AGEMAMI; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0004622; F:lysophospholipase activity; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0046470; P:phosphatidylcholine metabolic process; IEA:InterPro.
DR   CDD; cd00038; CAP_ED; 1.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR001423; LysoPLipase_patatin_CS.
DR   InterPro; IPR002641; PNPLA_dom.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   Pfam; PF00027; cNMP_binding; 1.
DR   Pfam; PF01734; Patatin; 1.
DR   SMART; SM00100; cNMP; 1.
DR   SUPFAM; SSF51206; SSF51206; 1.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
DR   PROSITE; PS51635; PNPLA; 1.
DR   PROSITE; PS01237; UPF0028; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Lipid degradation; Lipid metabolism.
FT   CHAIN           1..583
FT                   /note="Uncharacterized NTE family protein Mb2594"
FT                   /id="PRO_0000172537"
FT   DOMAIN          309..469
FT                   /note="PNPLA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           313..318
FT                   /note="GXGXXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           340..344
FT                   /note="GXSXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           456..458
FT                   /note="DGA/G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        342
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        456
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   BINDING         24..140
FT                   /ligand="a nucleoside 3',5'-cyclic phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58464"
SQ   SEQUENCE   583 AA;  62124 MW;  EC6EE062BAEC62F7 CRC64;
     MTTARRRPKR RGTDARTALR NVPILADIDD EQLERLATTV ERRHVPANQW LFHAGEPADS
     IYIVDSGRFV AVAPEGHVFA EMASGDSIGD LGVIAGAARS AGVRALRDGV VWRIAAETFT
     DMLEATPLLQ SAMLRAMARM LRQSRPAKTA RRPRVIGVVS NGDTAAAPMV DAIATSLDSH
     GRTAVIAPPV ETTSAVQEYD ELVEAFSETL DRAERSNDWV LVVADRGAGD LWRHYVSAQS
     DRLVVLVDQR YPPDAVDSLA TQRPVHLITC LAEPDPSWWD RLAPVSHHPA NSDGFGALAR
     RIAGRSLGLV MAGGGARGLA HFGVYQELTE AGVVIDRFGG TSSGAIASAA FALGMDAGDA
     IAAAREFIAG SDPLGDYTIP ISALTRGGRV DRLVQGFFGN TLIEHLPRGF FSVSADMITG
     DQIIHRRGSV SGAVRASISI PGLIPPVHNG EQLLVDGGLL NNLPANVMCA DTDGEVICVD
     LRRTFVPSKG FGLLPPIVTP PGLLRRLLTG TDNALPPLQE TLLRAFDLAA STANLRELPR
     VAAIIEPDVS KIGVLNFKQI DAALEAGRMA ARAALQAQPD LVR