ID   Y263_MYCGE              Reviewed;         291 AA.
AC   P47505; Q49216;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Putative phosphatase MG263;
DE            EC=3.1.3.-;
GN   OrderedLocusNames=MG263;
OS   Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS   (Mycoplasmoides genitalium).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=243273;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA   Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA   Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA   Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA   Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA   Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA   Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT   "The minimal gene complement of Mycoplasma genitalium.";
RL   Science 270:397-403(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 164-276.
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA   Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT   "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL   J. Bacteriol. 175:7918-7930(1993).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. Cof family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L43967; AAC71485.1; -; Genomic_DNA.
DR   EMBL; U01764; AAD10580.1; -; Genomic_DNA.
DR   PIR; A64229; A64229.
DR   RefSeq; WP_009885894.1; NZ_AAGX01000009.1.
DR   AlphaFoldDB; P47505; -.
DR   SMR; P47505; -.
DR   STRING; 243273.MG_263; -.
DR   PRIDE; P47505; -.
DR   EnsemblBacteria; AAC71485; AAC71485; MG_263.
DR   KEGG; mge:MG_263; -.
DR   eggNOG; COG0561; Bacteria.
DR   HOGENOM; CLU_044146_0_3_14; -.
DR   OMA; FPSARIY; -.
DR   OrthoDB; 1374424at2; -.
DR   BioCyc; MGEN243273:G1GJ2-319-MON; -.
DR   Proteomes; UP000000807; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR   GO; GO:0016791; F:phosphatase activity; IBA:GO_Central.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR000150; Cof.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR006379; HAD-SF_hydro_IIB.
DR   InterPro; IPR023214; HAD_sf.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR00099; Cof-subfamily; 1.
DR   TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR   PROSITE; PS01228; COF_1; 1.
DR   PROSITE; PS01229; COF_2; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Reference proteome.
FT   CHAIN           1..291
FT                   /note="Putative phosphatase MG263"
FT                   /id="PRO_0000054437"
FT   ACT_SITE        11
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         11
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         12
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
FT   BINDING         13
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         60..61
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
FT   BINDING         217
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
FT   BINDING         241
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         244
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        170
FT                   /note="L -> S (in Ref. 2; AAD10580)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        190
FT                   /note="I -> R (in Ref. 2; AAD10580)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   291 AA;  33423 MW;  708EFFEC6158F4B4 CRC64;
     MKNEIKYLYS DLDGTIVSWN PKTEFVYQNK SYKNFHEVSD ATISAFYRLQ QKGIKVGIVT
     GRDYCRVLWL EKQLRTGLPT ITLDGAIIFY QNEILSQTYL DDRFIEGINN IVKRFPEAAY
     KLNSGWISYF TKNPSVIFEI DYAFLGYFNP NTKLQKKFID STENWDLNKL KVNQVYFDID
     TCPLAMQKEI IELISVSDVN AKIYEHSMYI IKNGVSKASA LQSLNQFAIP ITKDNTIVCG
     DGDNDIEMMQ WAKHSVSLIG SNPKCFALAK YHTDSVDNDG IANWIEKNLL C