ID   Y264_METEX              Reviewed;         236 AA.
AC   Q8GEK8;
DT   19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Putative (5-formylfuran-3-yl)methyl phosphate synthase {ECO:0000250|UniProtKB:Q58499};
DE            EC=4.2.3.153 {ECO:0000250|UniProtKB:Q58499};
DE   AltName: Full=4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase {ECO:0000250|UniProtKB:Q58499};
DE            Short=4-HFC-P synthase {ECO:0000250|UniProtKB:Q58499};
OS   Methylorubrum extorquens (Methylobacterium dichloromethanicum)
OS   (Methylobacterium extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=408;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Marx C.J., Lidstrom M.E., Chistoserdova L.;
RT   "Metabolic module approach in genome analysis reveals that Burkholderia
RT   fungorum LB400 possesses tetrahydromethanopterin-linked formaldehyde
RT   oxidation functions.";
RL   Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of 4-(hydroxymethyl)-2-
CC       furancarboxaldehyde phosphate (4-HFC-P) from two molecules of
CC       glyceraldehyde-3-P (GA-3-P). {ECO:0000250|UniProtKB:Q58499}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-
CC         furancarboxaldehyde phosphate + 2 H2O + phosphate;
CC         Xref=Rhea:RHEA:43536, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:59776, ChEBI:CHEBI:83407; EC=4.2.3.153;
CC         Evidence={ECO:0000250|UniProtKB:Q58499};
CC   -!- SIMILARITY: Belongs to the MfnB family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY117134; AAM77053.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8GEK8; -.
DR   SMR; Q8GEK8; -.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR007565; 4HFCP_synth.
DR   Pfam; PF04476; 4HFCP_synth; 1.
DR   PIRSF; PIRSF015957; UCP015957; 1.
PE   3: Inferred from homology;
KW   Lyase; Schiff base.
FT   CHAIN           1..236
FT                   /note="Putative (5-formylfuran-3-yl)methyl phosphate
FT                   synthase"
FT                   /id="PRO_0000134869"
FT   ACT_SITE        38
FT                   /note="Schiff-base intermediate with substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q58499"
FT   ACT_SITE        94
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q58499"
SQ   SEQUENCE   236 AA;  23392 MW;  FBD4A573454D42BF CRC64;
     MSDIVSISSA RPRLLVSVRG PDEALTALRA GADLIDAKDP ERGALGALPP ETVRAIVAGV
     GGRAVTSAVA GDGTGREIAA AIATIAATGV DFIKIAVGGA DDAALAEAAA QAPGRVIGVL
     FAEDDVAEDG PARLAAAGFV GAMIDTRGKS GTTLTSLMAA PQLAAFVAGC RTHGLMSGLA
     GSLGLGDIPV LARLDPDYLG FRGGLCRASD RRQALDGARV AQAVEAMRAG PRADAA