Y2650_BACO1
ID Y2650_BACO1 Reviewed; 489 AA.
AC A7LXT4;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 1.
DT 25-MAY-2022, entry version 48.
DE RecName: Full=IPT/TIG domain-containing protein BACOVA_02650;
DE Flags: Precursor;
GN ORFNames=BACOVA_02650;
OS Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 /
OS CCUG 4943 / NCTC 11153).
OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=411476;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC
RC 11153;
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Bacteroides ovatus (ATCC 8483).";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, AND PATHWAY.
RX PubMed=24463512; DOI=10.1038/nature12907;
RA Larsbrink J., Rogers T.E., Hemsworth G.R., McKee L.S., Tauzin A.S.,
RA Spadiut O., Klinter S., Pudlo N.A., Urs K., Koropatkin N.M., Creagh A.L.,
RA Haynes C.A., Kelly A.G., Cederholm S.N., Davies G.J., Martens E.C.,
RA Brumer H.;
RT "A discrete genetic locus confers xyloglucan metabolism in select human gut
RT Bacteroidetes.";
RL Nature 506:498-502(2014).
CC -!- FUNCTION: Polysaccharide-binding protein present at the surface of the
CC cell. Probably mediates xyloglucan-binding before xyloglucan transport
CC in the periplasm for degradation. {ECO:0000269|PubMed:24463512}.
CC -!- PATHWAY: Glucan metabolism; xyloglucan degradation.
CC {ECO:0000269|PubMed:24463512}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}. Note=Cell outer membrane localization is predicted by
CC analogy with the archetypal sus locus. {ECO:0000269|PubMed:24463512}.
CC -!- MISCELLANEOUS: Gut bacteria supply the human body with energy from
CC dietary polysaccharides through glycosidases that are absent in the
CC human genome. Xyloglucans are a ubiquitous family of highly branched
CC plant cell wall polysaccharides present in the vegetables we consume.
CC Enzymes involved in xyloglucan degradation mediate the conversion of
CC otherwise indigestible plant polysaccharides to short-chain fatty acids
CC (PubMed:24463512). {ECO:0000305|PubMed:24463512}.
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DR EMBL; AAXF02000049; EDO11441.1; -; Genomic_DNA.
DR RefSeq; WP_004298439.1; NZ_DS264579.1.
DR PDB; 5E7G; X-ray; 2.37 A; A=34-489.
DR PDB; 5E7H; X-ray; 1.57 A; A=230-489.
DR PDBsum; 5E7G; -.
DR PDBsum; 5E7H; -.
DR AlphaFoldDB; A7LXT4; -.
DR SMR; A7LXT4; -.
DR STRING; 411476.BACOVA_02650; -.
DR EnsemblBacteria; EDO11441; EDO11441; BACOVA_02650.
DR GeneID; 29452189; -.
DR eggNOG; ENOG502Z8MW; Bacteria.
DR HOGENOM; CLU_044175_0_0_10; -.
DR UniPathway; UPA01045; -.
DR Proteomes; UP000005475; Unassembled WGS sequence.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030247; F:polysaccharide binding; IDA:UniProtKB.
DR GO; GO:0085030; P:symbiotic process benefiting host; IDA:UniProtKB.
DR GO; GO:2000899; P:xyloglucan catabolic process; IDA:UniProtKB.
DR Gene3D; 2.60.40.10; -; 3.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR040475; SGBP_B_XBD.
DR Pfam; PF18329; SGBP_B_XBD; 1.
DR SUPFAM; SSF81296; SSF81296; 2.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Cell outer membrane; Lipoprotein;
KW Membrane; Palmitate; Repeat; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 28..489
FT /note="IPT/TIG domain-containing protein BACOVA_02650"
FT /id="PRO_0000425885"
FT DOMAIN 57..103
FT /note="IPT/TIG 1"
FT DOMAIN 136..204
FT /note="IPT/TIG 2"
FT DOMAIN 232..304
FT /note="IPT/TIG 3"
FT LIPID 28
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 28
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT STRAND 38..43
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 62..69
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 75..78
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 81..83
FT /evidence="ECO:0007829|PDB:5E7G"
FT HELIX 87..89
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 92..98
FT /evidence="ECO:0007829|PDB:5E7G"
FT TURN 106..108
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 115..119
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 124..128
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 137..142
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 152..158
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 163..171
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 189..191
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 195..201
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 208..218
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 220..226
FT /evidence="ECO:0007829|PDB:5E7G"
FT STRAND 233..238
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 246..253
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 260..263
FT /evidence="ECO:0007829|PDB:5E7H"
FT TURN 264..266
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 267..269
FT /evidence="ECO:0007829|PDB:5E7H"
FT HELIX 271..273
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 280..286
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 297..301
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 304..307
FT /evidence="ECO:0007829|PDB:5E7H"
FT HELIX 315..317
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 318..320
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 322..330
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 334..337
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 340..342
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 348..358
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 360..371
FT /evidence="ECO:0007829|PDB:5E7H"
FT TURN 377..379
FT /evidence="ECO:0007829|PDB:5E7H"
FT HELIX 386..388
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 389..399
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 405..410
FT /evidence="ECO:0007829|PDB:5E7H"
FT HELIX 414..416
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 418..421
FT /evidence="ECO:0007829|PDB:5E7H"
FT TURN 426..428
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 434..442
FT /evidence="ECO:0007829|PDB:5E7H"
FT HELIX 443..445
FT /evidence="ECO:0007829|PDB:5E7H"
FT HELIX 452..456
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 462..469
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 471..473
FT /evidence="ECO:0007829|PDB:5E7H"
FT STRAND 475..488
FT /evidence="ECO:0007829|PDB:5E7H"
SQ SEQUENCE 489 AA; 53393 MW; ED64725F355249E8 CRC64;
MKSIYKYLDT RLFLIGLLVL PFLAVVSCQN DDDDAIPVIH YIRVTDPAKA DSTFTDVNPG
TMIVVVGEHL GGTQKVYIND QEVSFNRNYV TSTSIILTVP NELELTGQNP ELKGEIRIET
EHGVAAYNMH VLSPAPYITR ISATYPIKPG DQMTVIGGNF YEVQAVYLST EQPAKDGTRP
VDVQEITNYE VNNKYSQITL TAPANLLEEG YLVVECYTSS AVTEFKKNGP KPVVTAVSST
MPVVGSTVTI TGQNFIEVSR VNINGEFDIP VGDITTSNTF DEISFVLPQA PTQSGHISVT
AIGGTVESAE IFYPLENVIL NYDGIGSHVW GDCSFVVADG SSAPYVSNGT CLGITGTVSA
SNYWWKQSYS NAQWVNTSII PGNIPIDDLK LQFECFVKEV FTGPVFQIAM CENFDAALNG
YVPVSSFTGK TETGKWMQCS VSLSSVVADA TYQDFLNRNS THIGVYATNP GSSQATIEVY
FDNFRIVRK
