Y2706_ARATH
ID Y2706_ARATH Reviewed; 365 AA.
AC O80719; Q27GL0; Q2V401; Q3EBE8;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Probable receptor-like protein kinase At2g47060;
DE EC=2.7.11.1;
GN OrderedLocusNames=At2g47060; ORFNames=F14M4.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Southwick A., Karlin-Neumann G., Nguyen M., Lam B., Miranda M., Palm C.J.,
RA Theologis A., Ecker J., Davis R.W.;
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- INTERACTION:
CC O80719; Q39023: MPK3; NbExp=2; IntAct=EBI-4436376, EBI-349526;
CC O80719; Q39026: MPK6; NbExp=3; IntAct=EBI-4436376, EBI-349548;
CC O80719; Q9LSF1: OXI1; NbExp=4; IntAct=EBI-4436376, EBI-8574783;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=O80719-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O80719-2; Sequence=VSP_038437, VSP_038438;
CC Name=3;
CC IsoId=O80719-3; Sequence=VSP_038439;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR EMBL; AC004411; AAC34243.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10793.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10794.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10795.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10796.1; -; Genomic_DNA.
DR EMBL; AY080876; AAL87347.1; -; mRNA.
DR EMBL; AY114044; AAM45092.1; -; mRNA.
DR EMBL; AF325090; AAK17158.1; -; mRNA.
DR PIR; T02181; T02181.
DR RefSeq; NP_001031552.1; NM_001036475.1. [O80719-2]
DR RefSeq; NP_001031553.1; NM_001036476.1. [O80719-3]
DR RefSeq; NP_182229.1; NM_130274.4. [O80719-1]
DR RefSeq; NP_850467.2; NM_180136.3. [O80719-1]
DR AlphaFoldDB; O80719; -.
DR SMR; O80719; -.
DR BioGRID; 4655; 9.
DR IntAct; O80719; 5.
DR MINT; O80719; -.
DR STRING; 3702.AT2G47060.4; -.
DR SwissPalm; O80719; -.
DR PRIDE; O80719; -.
DR EnsemblPlants; AT2G47060.1; AT2G47060.1; AT2G47060. [O80719-1]
DR EnsemblPlants; AT2G47060.2; AT2G47060.2; AT2G47060. [O80719-1]
DR EnsemblPlants; AT2G47060.3; AT2G47060.3; AT2G47060. [O80719-2]
DR EnsemblPlants; AT2G47060.4; AT2G47060.4; AT2G47060. [O80719-3]
DR GeneID; 819320; -.
DR Gramene; AT2G47060.1; AT2G47060.1; AT2G47060. [O80719-1]
DR Gramene; AT2G47060.2; AT2G47060.2; AT2G47060. [O80719-1]
DR Gramene; AT2G47060.3; AT2G47060.3; AT2G47060. [O80719-2]
DR Gramene; AT2G47060.4; AT2G47060.4; AT2G47060. [O80719-3]
DR KEGG; ath:AT2G47060; -.
DR Araport; AT2G47060; -.
DR TAIR; locus:2041339; AT2G47060.
DR eggNOG; KOG1187; Eukaryota.
DR InParanoid; O80719; -.
DR OMA; FITTINH; -.
DR OrthoDB; 684563at2759; -.
DR PhylomeDB; O80719; -.
DR PRO; PR:O80719; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80719; baseline and differential.
DR Genevisible; O80719; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR GO; GO:0006979; P:response to oxidative stress; IEP:TAIR.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Kinase; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Serine/threonine-protein kinase;
KW Transferase.
FT CHAIN 1..365
FT /note="Probable receptor-like protein kinase At2g47060"
FT /id="PRO_0000389471"
FT DOMAIN 73..353
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 18..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 203
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 79..87
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 101
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 145
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 207
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 237
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 238
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 243
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 251
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT VAR_SEQ 1..99
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038437"
FT VAR_SEQ 100..121
FT /note="IKKLDSNKQPDNEFLAQVSMVS -> MICLQRLKSWILTNSLTMNSLL (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038438"
FT VAR_SEQ 323
FT /note="K -> KVRNQTFHNLRLCLRFRLHSLFLTSSYGDDDSQ (in isoform
FT 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_038439"
SQ SEQUENCE 365 AA; 39931 MW; ACAFEC6C79E77CAB CRC64;
MSCFGCCGED DDMHKTADYG GRHNQAKHFP PGNDARHHQA SETAQKGPPV VKLQPIEVPI
IPFSELKEAT DDFGSNSLIG EGSYGRVYYG VLNNDLPSAI KKLDSNKQPD NEFLAQVSMV
SRLKHDNFVQ LLGYCVDGNS RILSYEFANN GSLHDILHGR KGVKGAQPGP VLSWYQRVKI
AVGAARGLEY LHEKANPHII HRDIKSSNVL LFEDDVAKIA DFDLSNQAPD MAARLHSTRV
LGTFGYHAPE YAMTGQLNAK SDVYSFGVVL LELLTGRKPV DHRLPRGQQS LVTWATPKLS
EDKVKQCVDA RLGGDYPPKA VAKLAAVAAL CVQYEADFRP NMSIVVKALQ PLLNARAVAP
GEGVH
