ID   Y2743_MYCTU             Reviewed;         270 AA.
AC   I6YA50;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Putative envelope-preserving system protein Rv2743c {ECO:0000305};
GN   OrderedLocusNames=Rv2743c {ECO:0000312|EMBL:CCP45542.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   FUNCTION, INTERACTION WITH PSPA AND RV2742C, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=25899163; DOI=10.1111/mmi.13037;
RA   Datta P., Ravi J., Guerrini V., Chauhan R., Neiditch M.B., Shell S.S.,
RA   Fortune S.M., Hancioglu B., Igoshin O.A., Gennaro M.L.;
RT   "The Psp system of Mycobacterium tuberculosis integrates envelope stress-
RT   sensing and envelope-preserving functions.";
RL   Mol. Microbiol. 97:408-422(2015).
CC   -!- FUNCTION: Involved in preservation of envelope integrity and tolerance
CC       to surface stress (PubMed:25899163). Reverses the inhibitory effect of
CC       PspA on ClgR activity (PubMed:25899163). Facilitates intracellular
CC       growth of M.tuberculosis (PubMed:25899163).
CC       {ECO:0000269|PubMed:25899163}.
CC   -!- SUBUNIT: Interacts with PspA and Rv2742c.
CC       {ECO:0000269|PubMed:25899163}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is regulated by ClgR.
CC       {ECO:0000269|PubMed:25899163}.
CC   -!- DISRUPTION PHENOTYPE: The Rv2743c-Rv2742c double mutation reduces
CC       intracellular ATP levels under surface-stress conditions to less than
CC       60% of wild-type levels (PubMed:25899163). Inactivation of Rv2743c
CC       reduces mprA and sigB expression to 20% and 10% of wild-type levels,
CC       respectively, after treatment with SDS (PubMed:25899163). Inactivation
CC       increases M.tuberculosis susceptibility to the intramacrophage
CC       environment (PubMed:25899163). {ECO:0000269|PubMed:25899163}.
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DR   EMBL; AL123456; CCP45542.1; -; Genomic_DNA.
DR   RefSeq; NP_217259.1; NC_000962.3.
DR   RefSeq; WP_003414026.1; NZ_NVQJ01000020.1.
DR   AlphaFoldDB; I6YA50; -.
DR   STRING; 83332.Rv2743c; -.
DR   PaxDb; I6YA50; -.
DR   PRIDE; I6YA50; -.
DR   DNASU; 887779; -.
DR   GeneID; 45426730; -.
DR   GeneID; 887779; -.
DR   KEGG; mtu:Rv2743c; -.
DR   PATRIC; fig|83332.111.peg.3054; -.
DR   TubercuList; Rv2743c; -.
DR   eggNOG; ENOG5034BWG; Bacteria.
DR   OMA; AAWGWFA; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Membrane; Reference proteome; Stress response; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..270
FT                   /note="Putative envelope-preserving system protein Rv2743c"
FT                   /id="PRO_0000450868"
FT   TRANSMEM        50..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   270 AA;  28962 MW;  080418F9C798EC38 CRC64;
     MAVKAGQRRP WRSLLQRGVD TAGDLADLVA QKISVAIDPR ARLLRRRRRA LRWGLVFTAG
     CLLWGLVTAL LAAWGWFTSL LVITGTIAVT QAIPATLLLL RYRWLRSEPL PVRRPASVRR
     LPPPGSAARP AMSALGASER GFFSLLGVME RGAMLPADEI RDLTAAANQT SAAMVATAAE
     VVSMERAVQC SAASRSYLVP TINAFTAQLS TGVRQYNEMV TAAAQLVSSA NGAGGAGPGQ
     QRYREELAGA TDRLVAWAQA FDELGGLPRR