ID   Y2782_MYCTU             Reviewed;         438 AA.
AC   P9WHT5; L0TAV3; O33324; P0A5S8;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=Uncharacterized zinc protease Rv2782c;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=Rv2782c; ORFNames=MTV002.47c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M16 family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45581.1; -; Genomic_DNA.
DR   PIR; E70883; E70883.
DR   RefSeq; NP_217298.1; NC_000962.3.
DR   RefSeq; WP_003414119.1; NZ_NVQJ01000020.1.
DR   AlphaFoldDB; P9WHT5; -.
DR   SMR; P9WHT5; -.
DR   STRING; 83332.Rv2782c; -.
DR   PaxDb; P9WHT5; -.
DR   DNASU; 888470; -.
DR   GeneID; 888470; -.
DR   KEGG; mtu:Rv2782c; -.
DR   TubercuList; Rv2782c; -.
DR   eggNOG; COG0612; Bacteria.
DR   OMA; IDVVCDM; -.
DR   PhylomeDB; P9WHT5; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 1.
DR   SUPFAM; SSF63411; SSF63411; 2.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW   Zinc.
FT   CHAIN           1..438
FT                   /note="Uncharacterized zinc protease Rv2782c"
FT                   /id="PRO_0000074423"
FT   ACT_SITE        62
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT   BINDING         59
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT   BINDING         63
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT   BINDING         139
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
SQ   SEQUENCE   438 AA;  47072 MW;  C16707238706DE71 CRC64;
     MPRRSPADPA AALAPRRTTL PGGLRVVTEF LPAVHSASVG VWVGVGSRDE GATVAGAAHF
     LEHLLFKSTP TRSAVDIAQA MDAVGGELNA FTAKEHTCYY AHVLGSDLPL AVDLVADVVL
     NGRCAADDVE VERDVVLEEI AMRDDDPEDA LADMFLAALF GDHPVGRPVI GSAQSVSVMT
     RAQLQSFHLR RYTPERMVVA AAGNVDHDGL VALVREHFGS RLVRGRRPVA PRKGTGRVNG
     SPRLTLVSRD AEQTHVSLGI RTPGRGWEHR WALSVLHTAL GGGLSSRLFQ EVRETRGLAY
     SVYSALDLFA DSGALSVYAA CLPERFADVM RVTADVLESV ARDGITEAEC GIAKGSLRGG
     LVLGLEDSSS RMSRLGRSEL NYGKHRSIEH TLRQIEQVTV EEVNAVARHL LSRRYGAAVL
     GPHGSKRSLP QQLRAMVG