ID   Y281_BUCAI              Reviewed;         307 AA.
AC   P57368;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Uncharacterized transporter BU281;
GN   OrderedLocusNames=BU281;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the EamA transporter family. {ECO:0000305}.
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DR   EMBL; BA000003; BAB12991.1; -; Genomic_DNA.
DR   RefSeq; NP_240105.1; NC_002528.1.
DR   RefSeq; WP_010896043.1; NC_002528.1.
DR   AlphaFoldDB; P57368; -.
DR   SMR; P57368; -.
DR   STRING; 107806.10038956; -.
DR   TCDB; 2.A.7.3.23; the drug/metabolite transporter (dmt) superfamily.
DR   EnsemblBacteria; BAB12991; BAB12991; BAB12991.
DR   KEGG; buc:BU281; -.
DR   PATRIC; fig|107806.10.peg.291; -.
DR   eggNOG; COG0697; Bacteria.
DR   HOGENOM; CLU_033863_5_3_6; -.
DR   OMA; FKGLDPM; -.
DR   BioCyc; BAPH107806:GBZJ-276-MON; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..307
FT                   /note="Uncharacterized transporter BU281"
FT                   /id="PRO_0000108189"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        214..234
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        267..287
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          15..139
FT                   /note="EamA 1"
FT   DOMAIN          161..287
FT                   /note="EamA 2"
SQ   SEQUENCE   307 AA;  35006 MW;  5D1851AECF6EA6CE CRC64;
     MNKILVIILF SLVSLTWGTT WIAMKIATET IPPFFATGMR FLVASPILII LAYFTKTPLL
     FPYGQRWFQL IISIFYFSIP FALMLYGGMY VSSSVASIIF SSMPVAVLTV SFLYLKKKLF
     LTQKIGMFIS LITLLTILLI ELESQCFFQW KGILALLFAL FSHAFIYSEC QKKCLNVSVI
     TFNALPSLLS GILLSTTSWF IESPHINTFS NRSILAIFYL GDFSGIFGIL SYFYLQQKVS
     AFYASTVFLI FPVIAGFLEN YIYKNAILLC EIWFIFPLII GILLTLIPVN YLKKIRNKIT
     RLSCLLF