ID   Y2859_MYCTU             Reviewed;         308 AA.
AC   O33341; L0TAV0;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Putative glutamine amidotransferase Rv2859c;
DE            EC=2.4.2.-;
GN   OrderedLocusNames=Rv2859c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   PUPYLATION AT LYS-289, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20066036; DOI=10.1371/journal.pone.0008589;
RA   Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E.,
RA   Gygi S.P., Darwin K.H.;
RT   "Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium
RT   tuberculosis.";
RL   PLoS ONE 5:E8589-E8589(2010).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
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DR   EMBL; AL123456; CCP45660.1; -; Genomic_DNA.
DR   PIR; E70885; E70885.
DR   RefSeq; NP_217375.1; NC_000962.3.
DR   RefSeq; WP_003900586.1; NZ_NVQJ01000006.1.
DR   AlphaFoldDB; O33341; -.
DR   SMR; O33341; -.
DR   STRING; 83332.Rv2859c; -.
DR   PaxDb; O33341; -.
DR   DNASU; 887495; -.
DR   GeneID; 887495; -.
DR   KEGG; mtu:Rv2859c; -.
DR   TubercuList; Rv2859c; -.
DR   eggNOG; COG2071; Bacteria.
DR   InParanoid; O33341; -.
DR   OMA; PTYHHQA; -.
DR   PhylomeDB; O33341; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0033969; F:gamma-glutamyl-gamma-aminobutyrate hydrolase activity; IBA:GO_Central.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006598; P:polyamine catabolic process; IBA:GO_Central.
DR   Gene3D; 3.40.50.880; -; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR011697; Peptidase_C26.
DR   InterPro; IPR044668; PuuD-like.
DR   PANTHER; PTHR43235; PTHR43235; 1.
DR   Pfam; PF07722; Peptidase_C26; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   PROSITE; PS51273; GATASE_TYPE_1; 1.
PE   1: Evidence at protein level;
KW   Glutamine amidotransferase; Isopeptide bond; Reference proteome;
KW   Transferase; Ubl conjugation.
FT   CHAIN           1..308
FT                   /note="Putative glutamine amidotransferase Rv2859c"
FT                   /id="PRO_0000396115"
FT   DOMAIN          78..301
FT                   /note="Glutamine amidotransferase type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00605"
FT   REGION          1..62
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        177
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00605"
FT   ACT_SITE        277
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00605"
FT   ACT_SITE        279
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00605"
FT   CROSSLNK        289
FT                   /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT                   with Q-Cter in protein Pup)"
FT                   /evidence="ECO:0000269|PubMed:20066036"
SQ   SEQUENCE   308 AA;  32442 MW;  81F131E4252DC93B CRC64;
     MDLSASRSDG GDPLRPASPR LRSPVSDGGD PLRPASPRLR SPVSDGGDPL RPASPRLRSP
     LGASRPVVGL TAYLEQVRTG VWDIPAGYLP ADYFEGITMA GGVAVLLPPQ PVDPESVGCV
     LDSLHALVIT GGYDLDPAAY GQEPHPATDH PRPGRDAWEF ALLRGALQRG MPVLGICRGT
     QVLNVALGGT LHQHLPDILG HSGHRAGNGV FTRLPVHTAS GTRLAELIGE SADVPCYHHQ
     AIDQVGEGLV VSAVDVDGVI EALELPGDTF VLAVQWHPEK SLDDLRLFKA LVDAASGYAG
     RQSQAEPR