ID   Y2885_MYCTO             Reviewed;         460 AA.
AC   P9WL36; L0TDU6; Q10809;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=Putative RNA-guided DNA endonuclease MT2953 {ECO:0000250|UniProtKB:Q7DF80};
DE            EC=3.1.21.- {ECO:0000250|UniProtKB:Q7DF80};
GN   OrderedLocusNames=MT2953;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: An RNA-guided dsDNA endonuclease. When guided by an RNA
CC       derived from the right-end element of its insertion sequence element
CC       (IS), cleaves DNA downstream of the transposon-associated motif (TAM).
CC       Cleaves supercoiled and linear DNA in a staggered manner 15-21 bases
CC       from the TAM yielding 5'-overhangs. Binds reRNA, an approximately 150
CC       nucleotide base sRNA derived from the 3' end of its own gene, the right
CC       end (RE) of the insertion sequence (IS) plus sequence downstream of the
CC       IS. {ECO:0000250|UniProtKB:Q7DF80}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the transposase 2
CC       family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the transposase 35
CC       family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK47278.1; -; Genomic_DNA.
DR   PIR; H70924; H70924.
DR   AlphaFoldDB; P9WL36; -.
DR   EnsemblBacteria; AAK47278; AAK47278; MT2953.
DR   KEGG; mtc:MT2953; -.
DR   PATRIC; fig|83331.31.peg.3190; -.
DR   HOGENOM; CLU_032903_2_1_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR   InterPro; IPR001959; Transposase_2.
DR   InterPro; IPR010095; Transposase_IS605_OrfB_C.
DR   InterPro; IPR021027; Transposase_put_HTH.
DR   Pfam; PF12323; HTH_OrfB_IS605; 1.
DR   Pfam; PF01385; OrfB_IS605; 1.
DR   Pfam; PF07282; OrfB_Zn_ribbon; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Endonuclease; Hydrolase; Metal-binding;
KW   Nuclease; Transposable element; Transposition; Zinc.
FT   CHAIN           1..460
FT                   /note="Putative RNA-guided DNA endonuclease MT2953"
FT                   /id="PRO_0000427547"
FT   REGION          415..460
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        224
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT   ACT_SITE        313
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT   ACT_SITE        399
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT   BINDING         372
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT   BINDING         375
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT   BINDING         389
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF80"
FT   BINDING         392
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q7DF80"
SQ   SEQUENCE   460 AA;  51284 MW;  1FCBEDED35C11FC4 CRC64;
     MMARLKVPEG WCVQAFRFTL NPTQTQAASL ARHFGARRKA FNWTVTALKA DIKAWRADGT
     ESAKPSLRVL RKRWNTVKDQ VCVNAQTGQV WWPECSKEAY ADGIAGAVDA YWNWQSCRAG
     KRAGKTVGVP RFKKKGRDAD RVCFTTGAMR VEPDRRHLTL PVIGTIRTYE NTRRVERLIA
     KGRARVLAIT VRRNGTRLDA SVRVLVQRPQ QRRVALPDSR VGVDVGVRRL ATVADAEGTV
     LEQVPNPRPL DAALRGLRRV SRARSRCTKG SRRYCERTTE LSRLHRRVND VRTHHLHVLT
     TRLAKTHGRI VVEGLDAAGM LRQKGLPGAR ARRRALSDAA LATPRRHLSY KTGWYGSSLV
     VADRWFPSSK TCHACRHVQD IGWDEKWQCD GCSITHQRDD NAAINLARYE EPPSVVGPVG
     AAVKRGADRK TGPGPAGGRE ARKATGHPAG EQPRDGVQVK