Y2963_MYCTU
ID Y2963_MYCTU Reviewed; 406 AA.
AC I6YET7;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Putative permease Rv2963 {ECO:0000305};
GN OrderedLocusNames=Rv2963 {ECO:0000312|EMBL:CCP45767.1},
GN RVBD_2963 {ECO:0000312|EMBL:AFN50945.1};
GN ORFNames=P425_03084 {ECO:0000312|EMBL:KBJ29879.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genome Sequencing Platform;
RA Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [5]
RP INDUCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21166899; DOI=10.1111/j.1365-2958.2010.07431.x;
RA Festa R.A., Jones M.B., Butler-Wu S., Sinsimer D., Gerads R., Bishai W.R.,
RA Peterson S.N., Darwin K.H.;
RT "A novel copper-responsive regulon in Mycobacterium tuberculosis.";
RL Mol. Microbiol. 79:133-148(2011).
RN [6]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=24549843; DOI=10.1128/mbio.00876-13;
RA Shi X., Festa R.A., Ioerger T.R., Butler-Wu S., Sacchettini J.C.,
RA Darwin K.H., Samanovic M.I.;
RT "The copper-responsive RicR regulon contributes to Mycobacterium
RT tuberculosis virulence.";
RL MBio 5:E00876-E00876(2014).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Repressed by RicR. Induced by copper.
CC {ECO:0000269|PubMed:21166899}.
CC -!- DISRUPTION PHENOTYPE: Mutant is slightly more resistant to copper.
CC Mutation does not attenuate growth in mice.
CC {ECO:0000269|PubMed:24549843}.
CC -!- SIMILARITY: Belongs to the UPF0718 family. {ECO:0000305}.
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DR EMBL; AL123456; CCP45767.1; -; Genomic_DNA.
DR EMBL; CP003248; AFN50945.1; -; Genomic_DNA.
DR EMBL; JLDD01000035; KBJ29879.1; -; Genomic_DNA.
DR RefSeq; NP_217479.1; NC_000962.3.
DR RefSeq; WP_003414924.1; NZ_NVQJ01000015.1.
DR AlphaFoldDB; I6YET7; -.
DR STRING; 83332.Rv2963; -.
DR PaxDb; I6YET7; -.
DR DNASU; 887263; -.
DR GeneID; 45426951; -.
DR GeneID; 887263; -.
DR KEGG; mtu:Rv2963; -.
DR KEGG; mtv:RVBD_2963; -.
DR PATRIC; fig|83332.111.peg.3301; -.
DR TubercuList; Rv2963; -.
DR eggNOG; COG0701; Bacteria.
DR OMA; MGKDAWW; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR005524; DUF318.
DR Pfam; PF03773; ArsP_1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..406
FT /note="Putative permease Rv2963"
FT /id="PRO_0000433101"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 406 AA; 43729 MW; DDA7B4134FFC4D80 CRC64;
MTSTKVEDRV TAAVLGAIGH ALALTASMTW EILWALILGF ALSAVVQAVV RRSTIVTLLG
DDRPRTLVIA TGLGAASSSC SYAAVALARS LFRKGANFTA AMAFEIGSTN LVVELGIILA
LLMGWQFTAA EFVGGPIMIL VLAVLFRLFV GARLIDAARE QAERGLAGSM EGHAAMDMSI
KREGSFWRRL LSPPGFTSIA HVFVMEWLAI LRDLILGLLI AGAIAAWVPE SFWQSFFLAN
HPAWSAVWGP IIGPIVAIVS FVCSIGNVPL AAVLWNGGIS FGGVIAFIFA DLLILPILNI
YRKYYGARMM LVLLGTFYAS MVVAGYLIEL LFGTTNLIPS QRSATVMTAE ISWNYTTWLN
VIFLVIAAAL VVRFITSGGL PMLRMMGGSP DAPHDHHDRH DDHLGH
