Y2979_STAA8
ID Y2979_STAA8 Reviewed; 619 AA.
AC Q2G222;
DT 14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=N-acetylmuramoyl-L-alanine amidase domain-containing protein SAOUHSC_02979;
DE Flags: Precursor;
GN OrderedLocusNames=SAOUHSC_02979;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=ISP479C;
RX PubMed=18621893; DOI=10.1128/jb.00300-08;
RA Siboo I.R., Chaffin D.O., Rubens C.E., Sullam P.M.;
RT "Characterization of the accessory Sec system of Staphylococcus aureus.";
RL J. Bacteriol. 190:6188-6196(2008).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=RN4220;
RX PubMed=20472795; DOI=10.1128/jb.01452-09;
RA Sibbald M.J., Winter T., van der Kooi-Pol M.M., Buist G., Tsompanidou E.,
RA Bosma T., Schafer T., Ohlsen K., Hecker M., Antelmann H., Engelmann S.,
RA van Dijl J.M.;
RT "Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in
RT Staphylococcus aureus.";
RL J. Bacteriol. 192:3788-3800(2010).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18621893,
CC ECO:0000269|PubMed:20472795}.
CC -!- INDUCTION: More protein is secreted in a secA2 mutant
CC (PubMed:18621893), while less protein is secreted in a secG or double
CC secG/secY2 mutant (PubMed:20472795) (at protein level).
CC {ECO:0000269|PubMed:18621893, ECO:0000269|PubMed:20472795}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the N-acetylmuramoyl-
CC L-alanine amidase 2 family. {ECO:0000305}.
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DR EMBL; CP000253; ABD31967.1; -; Genomic_DNA.
DR RefSeq; WP_001125611.1; NZ_LS483365.1.
DR RefSeq; YP_501429.1; NC_007795.1.
DR PDB; 5T1Q; X-ray; 2.15 A; A/B/C/D=261-619.
DR PDBsum; 5T1Q; -.
DR AlphaFoldDB; Q2G222; -.
DR SMR; Q2G222; -.
DR STRING; 1280.SAXN108_2915; -.
DR CAZy; GH73; Glycoside Hydrolase Family 73.
DR PRIDE; Q2G222; -.
DR EnsemblBacteria; ABD31967; ABD31967; SAOUHSC_02979.
DR GeneID; 3921462; -.
DR KEGG; sao:SAOUHSC_02979; -.
DR PATRIC; fig|93061.5.peg.2687; -.
DR eggNOG; COG1705; Bacteria.
DR eggNOG; COG3942; Bacteria.
DR HOGENOM; CLU_017855_1_0_9; -.
DR OMA; YNRMAQF; -.
DR PRO; PR:Q2G222; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004040; F:amidase activity; IEA:InterPro.
DR InterPro; IPR007921; CHAP_dom.
DR InterPro; IPR002901; MGlyc_endo_b_GlcNAc-like_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR Pfam; PF05257; CHAP; 1.
DR Pfam; PF01832; Glucosaminidase; 1.
DR SMART; SM00047; LYZ2; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS50911; CHAP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..619
FT /note="N-acetylmuramoyl-L-alanine amidase domain-containing
FT protein SAOUHSC_02979"
FT /id="PRO_0000414194"
FT DOMAIN 488..617
FT /note="Peptidase C51"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00048"
FT REGION 25..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 134..226
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 238..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 327..468
FT /note="N-acetylmuramoyl-L-alanine amidase"
FT COMPBIAS 35..83
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..162
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 177..226
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 238..260
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 269..273
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 284..286
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 288..290
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 297..299
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 312..314
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 318..338
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 342..353
FT /evidence="ECO:0007829|PDB:5T1Q"
FT TURN 354..357
FT /evidence="ECO:0007829|PDB:5T1Q"
FT TURN 359..361
FT /evidence="ECO:0007829|PDB:5T1Q"
FT TURN 363..365
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 378..388
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 391..400
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 405..418
FT /evidence="ECO:0007829|PDB:5T1Q"
FT TURN 424..427
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 428..430
FT /evidence="ECO:0007829|PDB:5T1Q"
FT TURN 432..434
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 438..445
FT /evidence="ECO:0007829|PDB:5T1Q"
FT TURN 446..449
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 455..465
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 468..471
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 473..475
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 479..481
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 482..485
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 513..522
FT /evidence="ECO:0007829|PDB:5T1Q"
FT TURN 523..525
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 535..537
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 538..544
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 548..552
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 558..561
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 565..567
FT /evidence="ECO:0007829|PDB:5T1Q"
FT TURN 570..572
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 574..581
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 587..596
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 599..605
FT /evidence="ECO:0007829|PDB:5T1Q"
FT HELIX 607..610
FT /evidence="ECO:0007829|PDB:5T1Q"
FT STRAND 613..617
FT /evidence="ECO:0007829|PDB:5T1Q"
SQ SEQUENCE 619 AA; 69253 MW; 36FABFC6B73AF256 CRC64;
MPKNKILIYL LSTTLVLPTL VSPTAYADTP QKDTTAKTTS HDSKKSNDDE TSKDTTSKDI
DKADKNNTSN QDNNDKKFKT IDDSTSDSNN IIDFIYKNLP QTNINQLLTK NKYDDNYSLT
TLIQNLFNLN SDISDYEQPR NGEKSTNDSN KNSDNSIKND TDTQSSKQDK ADNQKAPKSN
NTKPSTSNKQ PNSPKPTQPN QSNSQPASDD KANQKSSSKD NQSMSDSALD SILDQYSEDA
KKTQKDYASQ SKKDKNEKSN TKNPQLPTQD ELKHKSKPAQ SFNNDVNQKD TRATSLFETD
PSISNNDDSG QFNVVDSKDT RQFVKSIAKD AHRIGQDNDI YASVMIAQAI LESDSGRSAL
AKSPNHNLFG IKGAFEGNSV PFNTLEADGN QLYSINAGFR KYPSTKESLK DYSDLIKNGI
DGNRTIYKPT WKSEADSYKD ATSHLSKTYA TDPNYAKKLN SIIKHYQLTQ FDDERMPDLD
KYERSIKDYD DSSDEFKPFR EVSDSMPYPH GQCTWYVYNR MKQFGTSISG DLGDAHNWNN
RAQYRDYQVS HTPKRHAAVV FEAGQFGADQ HYGHVAFVEK VNSDGSIVIS ESNVKGLGII
SHRTINAAAA EELSYITGK
