ID   Y2996_MYCBO             Reviewed;         282 AA.
AC   Q7TXI6; A0A1R3Y2Q3; X2BMD1;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Uncharacterized oxidoreductase Mb2996;
DE            EC=1.-.-.-;
GN   OrderedLocusNames=BQ2027_MB2996;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND INDUCTION.
RC   STRAIN=BCG / Pasteur;
RX   PubMed=16006064; DOI=10.1016/j.femsle.2005.06.004;
RA   Dosanjh N.S., Rawat M., Chung J.-H., Av-Gay Y.;
RT   "Thiol specific oxidative stress response in Mycobacteria.";
RL   FEMS Microbiol. Lett. 249:87-94(2005).
CC   -!- INDUCTION: Induced in response to the thiol oxidant diamide.
CC       {ECO:0000269|PubMed:16006064}.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR   EMBL; LT708304; SIU01619.1; -; Genomic_DNA.
DR   RefSeq; NP_856641.1; NC_002945.3.
DR   RefSeq; WP_003415022.1; NC_002945.4.
DR   AlphaFoldDB; Q7TXI6; -.
DR   SMR; Q7TXI6; -.
DR   EnsemblBacteria; SIU01619; SIU01619; BQ2027_MB2996.
DR   GeneID; 45426960; -.
DR   PATRIC; fig|233413.5.peg.3293; -.
DR   OMA; KLWPTDQ; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   PANTHER; PTHR43827; PTHR43827; 1.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
PE   1: Evidence at protein level;
KW   Oxidoreductase.
FT   CHAIN           1..282
FT                   /note="Uncharacterized oxidoreductase Mb2996"
FT                   /id="PRO_0000380732"
FT   ACT_SITE        57
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         195..247
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   282 AA;  30387 MW;  DDAF65BE669D7E60 CRC64;
     MTGESGAAAA PSITLNDEHT MPVLGLGVAE LSDDETERAV SAALEIGCRL IDTAYAYGNE
     AAVGRAIAAS GVAREELFVT TKLATPDQGF TRSQEACRAS LDRLGLDYVD LYLIHWPAPP
     VGKYVDAWGG MIQSRGEGHA RSIGVSNFTA EHIENLIDLT FVTPAVNQIE LHPLLNQDEL
     RKANAQHTVV TQSYCPLALG RLLDNPTVTS IASEYVKTPA QVLLRWNLQL GNAVVVRSAR
     PERIASNFDV FDFELAAEHM DALGGLNDGT RVREDPLTYA GT