ID   Y3017_CLOK5             Reviewed;         311 AA.
AC   P38943; A5N1M9;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Uncharacterized transporter CKL_3017;
DE   AltName: Full=ORFY;
GN   OrderedLocusNames=CKL_3017;
OS   Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=431943;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8550525; DOI=10.1128/jb.178.3.871-880.1996;
RA   Soehling B., Gottschalk G.;
RT   "Molecular analysis of the anaerobic succinate degradation pathway in
RT   Clostridium kluyveri.";
RL   J. Bacteriol. 178:871-880(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8527 / DSM 555 / NCIMB 10680;
RX   PubMed=18218779; DOI=10.1073/pnas.0711093105;
RA   Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H.,
RA   Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F.,
RA   Hagemeier C., Thauer R.K., Gottschalk G.;
RT   "The genome of Clostridium kluyveri, a strict anaerobe with unique
RT   metabolic features.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the EamA transporter family. {ECO:0000305}.
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DR   EMBL; L21902; AAA92345.1; -; Genomic_DNA.
DR   EMBL; CP000673; EDK35025.1; -; Genomic_DNA.
DR   RefSeq; WP_012103360.1; NC_009706.1.
DR   AlphaFoldDB; P38943; -.
DR   SMR; P38943; -.
DR   STRING; 431943.CKL_3017; -.
DR   EnsemblBacteria; EDK35025; EDK35025; CKL_3017.
DR   KEGG; ckl:CKL_3017; -.
DR   eggNOG; COG0697; Bacteria.
DR   HOGENOM; CLU_033863_13_0_9; -.
DR   OMA; AVMWGLS; -.
DR   OrthoDB; 1515911at2; -.
DR   Proteomes; UP000002411; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..311
FT                   /note="Uncharacterized transporter CKL_3017"
FT                   /id="PRO_0000108199"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        219..239
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        265..285
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          12..141
FT                   /note="EamA 1"
FT   DOMAIN          166..292
FT                   /note="EamA 2"
SQ   SEQUENCE   311 AA;  33508 MW;  A5E7CBE9EC9ADF0B CRC64;
     MKKGYIFILL TAIFYSTQEI SGKMLAQKGA MDPFQVMMIV FLIGAIILLP MAVKDIKVKK
     LKLTGNDLGY LALCGILAVS ISMSMLQFAV TYTKASTAAV LFCTNAVFTI PFAYFILKEK
     IKGITIVSII VSLIGVVIIF NPAKVMEGIG GSRDLIGICF ALVAAVVWSL YTVISKKRIE
     IYGGYVFNCI SFFFGVIALL ILLVVTGRPI FSGITLNNIL VLLYMGIFIK AVGYICYLGA
     IKETSAVTAS TVFLIKPALA TVLAILILGE SIEVNVVIGI VFIIIGSIIN YSSNKKANDL
     KKVANTSSAE S