ID   Y3070_LYSEN             Reviewed;        1068 AA.
AC   A0A0S2DN66;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   17-FEB-2016, sequence version 1.
DT   03-AUG-2022, entry version 17.
DE   RecName: Full=Probable ATPase FE772_23070 {ECO:0000303|PubMed:35025633};
DE            EC=3.6.4.- {ECO:0000305|PubMed:35025633};
GN   ORFNames=FE772_23070 {ECO:0000312|EMBL:QCW28098.1},
GN   Ga0399710_4916 {ECO:0000303|PubMed:35025633},
GN   GLE_4746 {ECO:0000312|EMBL:ALN60087.1};
OS   Lysobacter enzymogenes.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Lysobacter.
OX   NCBI_TaxID=69;
RN   [1] {ECO:0000312|EMBL:ALN60087.1, ECO:0000312|Proteomes:UP000061569}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C3;
RX   PubMed=26597042; DOI=10.1186/s12864-015-2191-z;
RA   de Bruijn I., Cheng X., de Jager V., Exposito R.G., Watrous J., Patel N.,
RA   Postma J., Dorrestein P.C., Kobayashi D., Raaijmakers J.M.;
RT   "Comparative genomics and metabolic profiling of the genus Lysobacter.";
RL   BMC Genomics 16:991-991(2015).
RN   [2] {ECO:0000312|EMBL:QCW28098.1, ECO:0000312|Proteomes:UP000308311}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YC36;
RX   PubMed=31540995; DOI=10.1128/aem.01742-19;
RA   Feng T., Han Y., Li B., Li Z., Yu Y., Sun Q., Li X., Du L., Zhang X.H.,
RA   Wang Y.;
RT   "Interspecies and Intraspecies Signals Synergistically Regulate Lysobacter
RT   enzymogenes Twitching Motility.";
RL   Appl. Environ. Microbiol. 85:0-0(2019).
RN   [3]
RP   FUNCTION, AND MUTAGENESIS OF LYS-223.
RC   STRAIN=YC36;
RX   PubMed=35025633; DOI=10.1126/science.abj8432;
RA   Johnson A.G., Wein T., Mayer M.L., Duncan-Lowey B., Yirmiya E.,
RA   Oppenheimer-Shaanan Y., Amitai G., Sorek R., Kranzusch P.J.;
RT   "Bacterial gasdermins reveal an ancient mechanism of cell death.";
RL   Science 375:221-225(2022).
CC   -!- FUNCTION: Involved in defense against bacteriophages. When this
CC       probable 4 gene operon (bGSDM-FE772_23060-FE772_23065-FE772_23070) is
CC       inserted into E.coli it provides nearly 100-fold protection against
CC       phages T5 and T6 and about 8-fold against phage T4. The operon without
CC       bGSDM no longer protects against phage (PubMed:35025633). Probably a
CC       nucleotide hydrolase, possibly of ATP (Probable).
CC       {ECO:0000269|PubMed:35025633, ECO:0000305|PubMed:35025633}.
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DR   EMBL; CP013140; ALN60087.1; -; Genomic_DNA.
DR   EMBL; CP040656; QCW28098.1; -; Genomic_DNA.
DR   RefSeq; WP_057949283.1; NZ_CP040656.1.
DR   EnsemblBacteria; ALN60087; ALN60087; GLE_4746.
DR   KEGG; lez:GLE_4746; -.
DR   PATRIC; fig|69.6.peg.4680; -.
DR   OrthoDB; 1099662at2; -.
DR   Proteomes; UP000061569; Chromosome.
DR   Proteomes; UP000308311; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; ATP-binding; Hydrolase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..1068
FT                   /note="Probable ATPase FE772_23070"
FT                   /id="PRO_0000455582"
FT   BINDING         217..224
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000305"
FT   MUTAGEN         223
FT                   /note="K->A: 4-gene operon no longer protects against
FT                   phage."
FT                   /evidence="ECO:0000269|PubMed:35025633"
SQ   SEQUENCE   1068 AA;  116113 MW;  CD12FBC564350833 CRC64;
     MTPTVPELEF AQATAFESNV PPLFPGRAGP DDGADRLRLR LAVRGWARPL DLIAASNSPD
     DVMAILGALA AEVETAVQTR PNQWYLSLAA RKRELARRDD AQLRAAASAS VPGDDDDPVL
     HAMRIALADA EPTLSALGTD VLAALGNACD WLGERWRHAV GAQRIAGELA SRALDADLRR
     MTRDPMVGRS HRDTLARLIG FATAATQTPL SVAYVYGGGG AGKTTLLSFL QRDLSQRAEP
     VPVVRIDFDE PAIDPTRMVT LNIALVEQLA RSVPAVCDRA SDMLPALRDT ALVQHDAGLS
     RGGPRKRIKS SRPESMLKAE SVASQAASDE GSILYRLLAP DVVAGPILIV FDTAELVLAQ
     SDHVASSLVS WLGFLHNEAG ARDLRLVIAG RDPPDDPDLG HAANSLLSRL KDTGARIETP
     IGLPELDPAE AQQLLRNCGV DDPTAAAEAA AAVPGNPLLL RITADALLQG EAELRESVRR
     AHRDSRIDAD SARNYLLRRV VAHVRDPIAR PYVLAATYSP VVTAKLLEEV VIPAVDRSER
     EPGPGIPANK KAATAKAKRV FDALASTYWL TRQTLRSETV PFNREIRAFA LKLLAATPEG
     ALLERDVRQS AAIHHLRRRS ADDRALALYH LAVLGHPYTV PRDLASVQTV LRDVIEELPA
     DLRNRLAPPV GSVAPGVARG VARSSIDDMS DSDWQRYLEG DERSKRAGEG AQMVKADRAR
     EALDLYLARP TRPPGLPPTF VIQAQADLGE WDEGIADIDA ILEREADDWL ARKSIGPEAL
     SRIYWITRLA LQAYGRLSTP HAMLLRNASE TATGPGLSTL PALIAVAETM SGEPIMAGRM
     RSQALKTDAA GRTLLCPIHG HLPETIELFD AGIAVVQSDW RQRMIQLAPA RLIRANEAHL
     RDLQSRLDAL HAKPIAQVNQ LFNKMRTGIA VEPTTMAGLN SAVLLLRGLT PEFYRPLREA
     LLALCENGVA SPMMRHAVDP LFERMSIRPA EMEPATFYRR LSNNPNAWCT AFIVYADRCR
     LLPGLCESLA RYADSPKSRR IASSFLAWDK ALCRGTSSDW GQPAKTRK