ID   Y3087_MYCTO             Reviewed;         472 AA.
AC   P9WKB0; L0TBI7; O53304;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT3172;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT3172;
GN   OrderedLocusNames=MT3172;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK47508.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK47508.1; ALT_INIT; Genomic_DNA.
DR   PIR; C70853; C70853.
DR   RefSeq; WP_003906978.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKB0; -.
DR   SMR; P9WKB0; -.
DR   EnsemblBacteria; AAK47508; AAK47508; MT3172.
DR   KEGG; mtc:MT3172; -.
DR   PATRIC; fig|83331.31.peg.3418; -.
DR   HOGENOM; CLU_024186_4_2_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..472
FT                   /note="Putative diacyglycerol O-acyltransferase MT3172"
FT                   /id="PRO_0000427685"
FT   REGION          217..238
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        139
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   472 AA;  52597 MW;  AC03BDDB4970FCC0 CRC64;
     MRRLNGVDAL MLYLDGGSAY NHTLKISVLD PSTDPDGWSW PKARQMFEER AHLLPVFRLR
     YLPTPLGLHH PIWVEDPEFD LDAHVRRVVC PAPGGMAEFC ALVEQIYAHP LDRDRPLWQT
     WVVEGLDGGR VALVTLLHHA YSDGVGVLDM LAAFYNDTPD EAPVVAPPWE PPPLPSTRQR
     LGWALRDLPS RLGKIAPTVR AVRDRVRIER EFAKDGDRRV PPTFDRSAPP GPFQRGLSRS
     RRFSCESFPL AEVREVSKTL GVTINDVFLA CVAGAVRRYL ERCGSPPTDA MVATMPLAVT
     PAAERAHPGN YSSVDYVWLR ADIADPLERL HATHLAAEAT KQHFAQTKDA DVGAVVELLP
     ERLISGLARA NARTKGRFDT FKNVVVSNVP GPREPRYLGR WRVDQWFSTG QISHGATLNM
     TVWSYCDQFN LCVMADAVAV RNTWELLGGF RASHEELLAA ARAQATPKEM AT