ID   Y308_MYCGE              Reviewed;         410 AA.
AC   P52271;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Probable ATP-dependent RNA helicase MG308;
DE            EC=3.6.4.13;
GN   OrderedLocusNames=MG308;
OS   Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS   (Mycoplasmoides genitalium).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=243273;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA   Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA   Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA   Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA   Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA   Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA   Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT   "The minimal gene complement of Mycoplasma genitalium.";
RL   Science 270:397-403(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA   Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT   "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL   J. Bacteriol. 175:7918-7930(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
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DR   EMBL; L43967; AAC71530.1; -; Genomic_DNA.
DR   EMBL; U02200; AAD12489.1; -; Genomic_DNA.
DR   PIR; A64234; A64234.
DR   RefSeq; WP_010869422.1; NC_000908.2.
DR   AlphaFoldDB; P52271; -.
DR   SMR; P52271; -.
DR   STRING; 243273.MG_308; -.
DR   EnsemblBacteria; AAC71530; AAC71530; MG_308.
DR   KEGG; mge:MG_308; -.
DR   eggNOG; COG0513; Bacteria.
DR   HOGENOM; CLU_003041_1_3_14; -.
DR   OMA; RRFLHDC; -.
DR   OrthoDB; 626183at2; -.
DR   BioCyc; MGEN243273:G1GJ2-377-MON; -.
DR   Proteomes; UP000000807; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..410
FT                   /note="Probable ATP-dependent RNA helicase MG308"
FT                   /id="PRO_0000055112"
FT   DOMAIN          26..179
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          190..357
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           126..129
FT                   /note="DEID box"
FT   BINDING         39..46
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   410 AA;  47990 MW;  953E3751ED3CEED7 CRC64;
     MQFSSSIRQF LDKKRIVEFT KIQQAVFKLW PFQNIIGIAE TGSGKTFAYL LPLLDKINTS
     LDQPQAVIFV PTKELQWQII NILTEIKKYF KTFTFATSFS SKAQLIVSLL NEKYLFTSKV
     RYVVFDEIDM FLEQSSIQQW LECVHLFQKA KPLFAFFSAT LFNQQLQIIK KQVINTKVIN
     LHPKQWIHPL VKHFVVHLNT ENRFSGLLAL LKHHQNQQII VFCSNQKSLK QLTQLLSNNN
     ISFGSIYGSL TYQERKNNFT KATNNKLKLL VVSDLFSRGI DLNYFSVVIS WDLPKIDSFY
     IHRSGRVARL NSWGRSYLFW NDQNQSKLNK LIAKGIKFQN VSLTSNGDLK FLTENNQKTT
     KKPLSTLQIK KIKAIKAKYK KVKPNYKKYQ KQQIQNLLIN KKKPRSWKNF