ID   Y309_MYCGE              Reviewed;        1225 AA.
AC   P47551; Q49317;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2018, sequence version 2.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Uncharacterized lipoprotein MG309;
DE   Flags: Precursor;
GN   OrderedLocusNames=MG309;
OS   Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS   (Mycoplasmoides genitalium).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=243273;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA   Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA   Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA   Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA   Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA   Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA   Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT   "The minimal gene complement of Mycoplasma genitalium.";
RL   Science 270:397-403(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1138-1224.
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA   Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT   "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL   J. Bacteriol. 175:7918-7930(1993).
RN   [3]
RP   SEQUENCE REVISION TO 844, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=16407165; DOI=10.1073/pnas.0510013103;
RA   Glass J.I., Assad-Garcia N., Alperovich N., Yooseph S., Lewis M.R.,
RA   Maruf M., Hutchison C.A. III, Smith H.O., Venter J.C.;
RT   "Essential genes of a minimal bacterium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:425-430(2006).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- DISRUPTION PHENOTYPE: Probably essential, it was not disrupted in a
CC       global transposon mutagenesis study. {ECO:0000269|PubMed:16407165}.
CC   -!- SIMILARITY: Belongs to the MG307/MG309/MG338 family. {ECO:0000305}.
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DR   EMBL; L43967; AAC71531.2; -; Genomic_DNA.
DR   EMBL; U02200; AAD12488.1; -; Genomic_DNA.
DR   PIR; B64234; B64234.
DR   AlphaFoldDB; P47551; -.
DR   STRING; 243273.MG_309; -.
DR   EnsemblBacteria; AAC71531; AAC71531; MG_309.
DR   KEGG; mge:MG_309; -.
DR   eggNOG; ENOG5031Y88; Bacteria.
DR   HOGENOM; CLU_007912_0_0_14; -.
DR   OMA; MAIDGAN; -.
DR   Proteomes; UP000000807; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR022186; DUF3713.
DR   Pfam; PF12506; DUF3713; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           28..1225
FT                   /note="Uncharacterized lipoprotein MG309"
FT                   /id="PRO_0000014032"
FT   REGION          995..1014
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           28
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           28
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CONFLICT        1185
FT                   /note="L -> V (in Ref. 2; AAD12488)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1225 AA;  138375 MW;  41E7323E8C78998C CRC64;
     MKRFLHRVKW PLLLSSIAVS LGIVAVACAQ PNSRTIENLF RPSSAFTDKN DGSINATLYK
     ALENREGLTQ YLTMRLAPVL RNFYEENVDD DIKRNLRTFN TDTDNSFVNQ EQNLRNQYRG
     DYLVRLQTDI LDNTGGNQAN WKLRDVNNKI VDDFINKLFT KNFVEYVDKS VGVLSTPLKG
     LIENQSNWNN IKIQAKFVDK NKRLRINNDA VYAAIQDKLL DQFVTNENPN LVSRVVFTNE
     TPNDGFDNYF NPDLIKSPTP SYQFQVFNKY NQQDNSIKGA NGFHILASNL QSYVNTNNKT
     IDIPNKFSSD SGGKLLLKAS DMFDTFDPSF SAAFIQGYLA LQKKSKGAEQ TEYTKLEKDK
     SIIENFFVEN NSAKAAMKSA SSSSQTTTVH KTDLAKIFKE NETTKSTDVF KGEYQKKFSN
     ATSTSNSDSS NNSAIVDLKE LKKDNNSQPD LILARGKDGI HLMGVDGGGY YLSESGRDVN
     KQKQFLLFRA LQTKYGLIDT NTTYDFKLFD EVKKHFDKNR VLFLFNALFK LVDSKESNFL
     SFPQFKKFSD SIITVKNELK DLVESQYQQI VFNEVATAEN KVALKLAERN QPFIDNERNK
     QIWMNGLAAV LPYEQDSKTG HYNELGIYYK DIIDKVSSNT SNSNSNSNSS SSDPFSKKII
     DKLKENKKKV EAAVKKHVDE LKVSVIPSPQ YSQIILVDTK LSSDPRNTSL ALNLALNAVL
     SSDELQNTIR RDYFVNDDQF KQAIDLDKLT FKNWNSLNNE NWNIFKYTYL FDLFQKQANP
     SIFGNGVNES STDNKPKING VLDSLYNSLN LEERLDSNDL INYYSYLYTV QWLLKDNLKN
     LKQNLQAKLS RTTNSFLVWS LASDKDRNNT ASQAMSVSSS KSVLVKMANN VASQTNQDFT
     KQEQQNPNYV FGSSAYNWTN NKTPTVNSAA NDISSLYYTK NNGSSSTSLT LMQKSAQQTN
     NQQRRFGFHG IVTNTSSNNL PDAVRNRLFT SFVSQSEKSS SNGGQAQLQS TQSSGSNETI
     YKGALFSFGS LTKLIETIDN IPTQAEFDAL YNHLTSNLNI NVTGVDRSKS LQEQKTNLKN
     FANSNFNNTQ TVQLKQAQSK TNNSNFNDVF SRFEGYIGTN KTSNYSSYNF LQDNQIYHAV
     YAKQINLEDV SMLGSDSLNS TDSNNSKRLD LSLEEFLSTV ALEALNPNNQ TQAINALIAN
     AKNGLVRVGD NRLFSAISSQ WVRKF