ID   Y309_SYNY3              Reviewed;         473 AA.
AC   Q55914;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Uncharacterized methyltransferase slr0309;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=slr0309;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX   PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA   Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA   Sugiura M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT   from map positions 64% to 92% of the genome.";
RL   DNA Res. 2:153-166(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000305};
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; BA000022; BAA10677.1; -; Genomic_DNA.
DR   PIR; S76985; S76985.
DR   AlphaFoldDB; Q55914; -.
DR   SMR; Q55914; -.
DR   IntAct; Q55914; 3.
DR   STRING; 1148.1001797; -.
DR   PaxDb; Q55914; -.
DR   EnsemblBacteria; BAA10677; BAA10677; BAA10677.
DR   KEGG; syn:slr0309; -.
DR   eggNOG; COG1032; Bacteria.
DR   InParanoid; Q55914; -.
DR   OMA; YFTTNVV; -.
DR   PhylomeDB; Q55914; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.80.30.20; -; 1.
DR   InterPro; IPR006158; Cobalamin-bd.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR034466; Methyltransferase_Class_B.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   Pfam; PF02310; B12-binding; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SMART; SM00729; Elp3; 1.
DR   PROSITE; PS51332; B12_BINDING; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Methyltransferase;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..473
FT                   /note="Uncharacterized methyltransferase slr0309"
FT                   /id="PRO_0000157869"
FT   DOMAIN          1..141
FT                   /note="B12-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00666"
FT   DOMAIN          163..391
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         177
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         181
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         184
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   473 AA;  54477 MW;  A9B942A52426175C CRC64;
     MEHKPNEFVK RAMKIDIIIV YIQRYRKGHE VHFVPPLTGI HLAALTPAKH TVRVIHQQVE
     TVNLDSEADV IVLSFFSGFA PEAYRLATEY RKRGKITIAG GPHVTFAPEE ALRFVDSIVI
     GEGESVWEQI FQDIEQNTLQ QRYIGEAIPL TNIPTPRYDL LPPQYFIRRV VQATRGCPFT
     CSFCTVPVLN PGFRTRPVEA VLNDIRYNKF KWWWQRKTVW FWDDNLTANR PYIKELLRQM
     IPLRKWWLTQ ASMDITKDDE LLDLMRDSGC IGIFFGIESF GQESLEDAGK RQNKVAEYQG
     KIKKLHDRGI GVMAGFISGL DGDTPESIRD MANQLEEIGV DVPFLSILTP FKGTRSYSKM
     MDTDRLRSDL GWEFYNGYNV TFVPSKMTPA ELLKAHRDLW RSAFSLQAIF RRIGRSLFTL
     RGGAMMMCSI MNLFYGLKAL TNNEPISFEG TDEYKSIRET IDLIPYSRQN SLS