Y3115_MYCBO
ID Y3115_MYCBO Reviewed; 474 AA.
AC P67209; A0A1R3Y344; O53305; X2BML1;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Putative diacyglycerol O-acyltransferase Mb3115;
DE EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE AltName: Full=Putative triacylglycerol synthase Mb3115;
GN OrderedLocusNames=BQ2027_MB3115;
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000305}.
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DR EMBL; LT708304; SIU01740.1; -; Genomic_DNA.
DR RefSeq; NP_856760.1; NC_002945.3.
DR RefSeq; WP_003416079.1; NC_002945.4.
DR AlphaFoldDB; P67209; -.
DR SMR; P67209; -.
DR EnsemblBacteria; SIU01740; SIU01740; BQ2027_MB3115.
DR PATRIC; fig|233413.5.peg.3422; -.
DR OMA; GHPLWEC; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650; PTHR31650; 1.
DR Pfam; PF03007; WES_acyltransf; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW Transferase.
FT CHAIN 1..474
FT /note="Putative diacyglycerol O-acyltransferase Mb3115"
FT /id="PRO_0000222915"
FT ACT_SITE 135
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
SQ SEQUENCE 474 AA; 50887 MW; 36832D972BE3851A CRC64;
MTRINPIDLS FLLLERANRP NHMAAYTIFE KPKGQKSSFG PRLFDAYRHS QAAKPFNHKL
KWLGTDVAAW ETVEPDMGYH IRHLALPAPG SMQQFHETVS FLNTGLLDRG HPMWECYIID
GIERGRIAIL LKVHHALIDG EGGLRAMRNF LSDSPDDTTL AGPWMSAQGA DRPRRTPATV
SRRAQLQGQL QGMIKGLTKL PSGLFGVSAD AADLGAQALS LKARKASLPF TARRTLFNNT
AKSAARAYGN VELPLADVKA LAKATGTSVN DVVMTVIDDA LHHYLAEHQA STDRPLVAFM
PMSLREKSGE GGGNRVSAEL VPMGAPKASP VERLKEINAA TTRAKDKGRG MQTTSRQAYA
LLLLGSLTVA DALPLLGKLP SANVVISNMK GPTEQLYLAG APLVAFSGLP IVPPGAGLNV
TFASINTALC IAIGAAPEAV HEPSRLAELM QRAFTELQTE AGTTSPTTSK SRTP
